4CAS : Serial femtosecond crystallography structure of a photosynthetic reaction center

Experimental Technique/Method:X-RAY DIFFRACTION Resolution:3.5 Classification:PHOTOSYNTHESIS Release Date:2013-12-25 Deposition Date:2013-10-09 Revision Date:2014-01-08#2017-06-14#2017-11-15 Molecular Weight:146379.91 Macromolecule Type:Protein Residue Count:1212 Atom Site Count:9890 DOI:10.2210/pdb4cas/pdb Abstract: Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 Å resolution and determine its serial femtosecond crystallography structure to 3.5 Å resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.