@article{476eb1f7d6cd4d02a36b2c0f8a5232e6,
title = "A long non-coding RNA regulates cadherin transcription and susceptibility to Bt toxin Cry1Ac in pink bollworm, Pectinophora gossypiella",
abstract = "Extensive planting of transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) has spurred increasingly rapid evolution of resistance in pests. In the pink bollworm, Pectinophora gossypiella, a devastating global pest, resistance to Bt toxin Cry1Ac produced by transgenic cotton is linked with mutations in a gene (PgCad1) encoding a cadherin protein that binds Cry1Ac in the larval midgut. We previously reported a long non-coding RNA (lncRNA) in intron 20 of cadherin alleles associated with both resistance and susceptibility to Cry1Ac. Here we tested the hypothesis that reducing expression of this lncRNA decreases transcription of PgCad1 and susceptibility to Cry1Ac. Quantitative RT-PCR showed that feeding susceptible neonates small interfering RNAs (siRNAs) targeting this lncRNA but not PgCad1 decreased the abundance of transcripts of both the lncRNA and PgCad1. Moreover, neonates fed the siRNAs had lower susceptibility to Cry1Ac. The results imply that the lncRNA increases transcription of PgCad1 and susceptibility of pink bollworm to Cry1Ac. The results suggest that disruption of lncRNA expression could be a novel mechanism of pest resistance to Bt toxins.",
keywords = "Gene regulation, Genetically modified crop, Off-target effects, Pest control, Resistance, Small interfering RNAs (siRNAs)",
author = "Shengyun Li and Fiaz Hussain and Unnithan, {Gopalan C.} and Shuanglin Dong and Zain UlAbdin and Shaohua Gu and Mathew, {Lolita G.} and Fabrick, {Jeffrey A.} and Xinzhi Ni and Yves Carri{\`e}re and Tabashnik, {Bruce E.} and Xianchun Li",
note = "Funding Information: This work was supported by the USDA National Institute of Food and Agriculture ( Hatch Grant ARZT-1360890-H31-164 , Multi-state Grant ARZT-1370400-R31-168 , and Agriculture and Food Research Initiative Program Grant 2018-67013-27821 ), the National Natural Science Foundation of China ( 31772164 , 31401737 , and 31171874 ), the China Scholarship Council for a scholarship to Mr. Shengyun Li, and the Pakistan Higher Education Commission (HEC) for a IRSIP Fellowship ( 1-8/HEC/HRD/2016/5657 , PIN: IRSIP 31 Agri26, dated. April 01-2016) to Mr. Fiaz Hussain. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. USDA is an equal opportunity provider and employer. Funding Information: This work was supported by the USDA National Institute of Food and Agriculture (Hatch Grant ARZT-1360890-H31-164, Multi-state Grant ARZT-1370400-R31-168, and Agriculture and Food Research Initiative Program Grant 2018-67013-27821), the National Natural Science Foundation of China (31772164, 31401737, and 31171874), the China Scholarship Council for a scholarship to Mr. Shengyun Li, and the Pakistan Higher Education Commission (HEC) for a IRSIP Fellowship (1-8/HEC/HRD/2016/5657, PIN: IRSIP 31 Agri26, dated. April 01-2016) to Mr. Fiaz Hussain. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. USDA is an equal opportunity provider and employer. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = jul,
doi = "10.1016/j.pestbp.2019.04.007",
language = "English (US)",
volume = "158",
pages = "54--60",
journal = "Pesticide Biochemistry and Physiology",
issn = "0048-3575",
publisher = "Academic Press Inc.",
}