A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes

Jacob Knittel; Gayathri Srinivasan; Carlye Frisch; Nicholas Brookhouser; Sreedevi Raman; Albert Essuman; David A. Brafman

doi:10.1016/j.xpro.2022.101632

A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes

Jacob Knittel
, Gayathri Srinivasan
, Carlye Frisch
, Nicholas Brookhouser
, Sreedevi Raman
, Albert Essuman
, David A. Brafman

Engineering, Ira A. Fulton Schools of (IAFSE)

Research output: Contribution to journal › Article › peer-review

Abstract

Here, we describe a protocol for a microcarrier (MC)-based, large-scale generation and cryopreservation of human-induced pluripotent stem cell (hiPSC)-derived neurons and astrocytes. We also detail steps to isolate these populations with a high degree of purity. Finally, we describe how to cryopreserve these cell types while maintaining high levels of viability and preserving cellular function post-thaw. For complete details on the use and execution of this protocol, please refer to Brookhouser et al. (2021).

Original language	English (US)
Article number	101632
Journal	STAR Protocols
Volume	3
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2022.101632
State	Published - Sep 16 2022

Keywords

Cell differentiation
Neuroscience
Stem cells
Tissue engineering

ASJC Scopus subject areas

General Neuroscience
General Immunology and Microbiology
General Biochemistry, Genetics and Molecular Biology

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10.1016/j.xpro.2022.101632

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title = "A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes",

abstract = "Here, we describe a protocol for a microcarrier (MC)-based, large-scale generation and cryopreservation of human-induced pluripotent stem cell (hiPSC)-derived neurons and astrocytes. We also detail steps to isolate these populations with a high degree of purity. Finally, we describe how to cryopreserve these cell types while maintaining high levels of viability and preserving cellular function post-thaw. For complete details on the use and execution of this protocol, please refer to Brookhouser et al. (2021).",

keywords = "Cell differentiation, Neuroscience, Stem cells, Tissue engineering",

author = "Jacob Knittel and Gayathri Srinivasan and Carlye Frisch and Nicholas Brookhouser and Sreedevi Raman and Albert Essuman and Brafman, \{David A.\}",

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AU - Knittel, Jacob

AU - Srinivasan, Gayathri

AU - Frisch, Carlye

AU - Brookhouser, Nicholas

AU - Raman, Sreedevi

AU - Essuman, Albert

AU - Brafman, David A.

PY - 2022/9/16

Y1 - 2022/9/16

N2 - Here, we describe a protocol for a microcarrier (MC)-based, large-scale generation and cryopreservation of human-induced pluripotent stem cell (hiPSC)-derived neurons and astrocytes. We also detail steps to isolate these populations with a high degree of purity. Finally, we describe how to cryopreserve these cell types while maintaining high levels of viability and preserving cellular function post-thaw. For complete details on the use and execution of this protocol, please refer to Brookhouser et al. (2021).

AB - Here, we describe a protocol for a microcarrier (MC)-based, large-scale generation and cryopreservation of human-induced pluripotent stem cell (hiPSC)-derived neurons and astrocytes. We also detail steps to isolate these populations with a high degree of purity. Finally, we describe how to cryopreserve these cell types while maintaining high levels of viability and preserving cellular function post-thaw. For complete details on the use and execution of this protocol, please refer to Brookhouser et al. (2021).

KW - Cell differentiation

KW - Neuroscience

KW - Stem cells

KW - Tissue engineering

UR - https://www.scopus.com/pages/publications/85136240302

UR - https://www.scopus.com/pages/publications/85136240302#tab=citedBy

U2 - 10.1016/j.xpro.2022.101632

DO - 10.1016/j.xpro.2022.101632

M3 - Article

C2 - 36035791

SN - 2666-1667

VL - 3

JO - STAR Protocols

JF - STAR Protocols

IS - 3

M1 - 101632

ER -

A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes

Abstract

Keywords

ASJC Scopus subject areas

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