A miniaturized cyclic PCR device - Modeling and experiments

C. F. Chou; R. Changrani; P. Roberts; D. Sadler; J. Burdon; F. Zenhausern; S. Lin; A. Mulholland; N. Swami; R. Terbrueggen

doi:10.1016/S0167-9317(02)00450-1

A miniaturized cyclic PCR device - Modeling and experiments

C. F. Chou, R. Changrani, P. Roberts, D. Sadler, J. Burdon, F. Zenhausern, S. Lin, A. Mulholland, N. Swami, R. Terbrueggen

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

With the aid of thermal and fluidic modeling using CFDRC ACE + ™, we designed and fabricated the first miniaturized cyclic polymerase chain reaction (PCR) device in low-temperature cofired ceramics. The device comprises of a serpentine channel with different cross-sectional areas in different reactor zones to provide adequate residence time for the melting, annealing, and extension reaction to take place. This is in contrary to the thermal cycling in the batch PCR system. With a flow rate of 15 μl/min, the designed time to complete 30 PCR cycles is less than 40 min, given the total volume of the device 19 μl, provided an internal pump may be implemented to reduce the dead volume. We have demonstrated DNA amplification in this device, using an external peristaltic pump, and the PCR product was used with a DNA bioelectronic sensor chip (Motorola e-Sensors™) for genotyping experiment.

Original language	English (US)
Pages (from-to)	921-925
Number of pages	5
Journal	Microelectronic Engineering
Volume	61-62
DOIs	https://doi.org/10.1016/S0167-9317(02)00450-1
State	Published - Jul 2002

Keywords

Ceramics
Continuous-flow
Microfluidics
PCR
Thermal modeling

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Condensed Matter Physics
Surfaces, Coatings and Films
Electrical and Electronic Engineering

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10.1016/S0167-9317(02)00450-1

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title = "A miniaturized cyclic PCR device - Modeling and experiments",

abstract = "With the aid of thermal and fluidic modeling using CFDRC ACE + {\texttrademark}, we designed and fabricated the first miniaturized cyclic polymerase chain reaction (PCR) device in low-temperature cofired ceramics. The device comprises of a serpentine channel with different cross-sectional areas in different reactor zones to provide adequate residence time for the melting, annealing, and extension reaction to take place. This is in contrary to the thermal cycling in the batch PCR system. With a flow rate of 15 μl/min, the designed time to complete 30 PCR cycles is less than 40 min, given the total volume of the device 19 μl, provided an internal pump may be implemented to reduce the dead volume. We have demonstrated DNA amplification in this device, using an external peristaltic pump, and the PCR product was used with a DNA bioelectronic sensor chip (Motorola e-Sensors{\texttrademark}) for genotyping experiment.",

keywords = "Ceramics, Continuous-flow, Microfluidics, PCR, Thermal modeling",

author = "Chou, {C. F.} and R. Changrani and P. Roberts and D. Sadler and J. Burdon and F. Zenhausern and S. Lin and A. Mulholland and N. Swami and R. Terbrueggen",

year = "2002",

month = jul,

doi = "10.1016/S0167-9317(02)00450-1",

language = "English (US)",

volume = "61-62",

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T1 - A miniaturized cyclic PCR device - Modeling and experiments

AU - Chou, C. F.

AU - Changrani, R.

AU - Roberts, P.

AU - Sadler, D.

AU - Burdon, J.

AU - Zenhausern, F.

AU - Lin, S.

AU - Mulholland, A.

AU - Swami, N.

AU - Terbrueggen, R.

PY - 2002/7

Y1 - 2002/7

N2 - With the aid of thermal and fluidic modeling using CFDRC ACE + ™, we designed and fabricated the first miniaturized cyclic polymerase chain reaction (PCR) device in low-temperature cofired ceramics. The device comprises of a serpentine channel with different cross-sectional areas in different reactor zones to provide adequate residence time for the melting, annealing, and extension reaction to take place. This is in contrary to the thermal cycling in the batch PCR system. With a flow rate of 15 μl/min, the designed time to complete 30 PCR cycles is less than 40 min, given the total volume of the device 19 μl, provided an internal pump may be implemented to reduce the dead volume. We have demonstrated DNA amplification in this device, using an external peristaltic pump, and the PCR product was used with a DNA bioelectronic sensor chip (Motorola e-Sensors™) for genotyping experiment.

AB - With the aid of thermal and fluidic modeling using CFDRC ACE + ™, we designed and fabricated the first miniaturized cyclic polymerase chain reaction (PCR) device in low-temperature cofired ceramics. The device comprises of a serpentine channel with different cross-sectional areas in different reactor zones to provide adequate residence time for the melting, annealing, and extension reaction to take place. This is in contrary to the thermal cycling in the batch PCR system. With a flow rate of 15 μl/min, the designed time to complete 30 PCR cycles is less than 40 min, given the total volume of the device 19 μl, provided an internal pump may be implemented to reduce the dead volume. We have demonstrated DNA amplification in this device, using an external peristaltic pump, and the PCR product was used with a DNA bioelectronic sensor chip (Motorola e-Sensors™) for genotyping experiment.

KW - Ceramics

KW - Continuous-flow

KW - Microfluidics

KW - PCR

KW - Thermal modeling

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VL - 61-62

SP - 921

EP - 925

JO - Microelectronic Engineering

JF - Microelectronic Engineering

ER -

A miniaturized cyclic PCR device - Modeling and experiments

Abstract

Keywords

ASJC Scopus subject areas

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