A role for CITED2, a CBP/p300 interacting protein, in colon cancer cell invasion

Longchuan Bai; Juanita L. Merchant

doi:10.1016/j.febslet.2007.11.072

A role for CITED2, a CBP/p300 interacting protein, in colon cancer cell invasion

Longchuan Bai, Juanita L. Merchant

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

A thorough understanding of histone acetyltransferase CBP/p300-mediated regulation of gene expression and cell growth is essential to identify mechanisms relevant to the development of histone deacetylase (HDAC) inhibitor-based preventive and therapeutic strategies. We found that knockdown of CBP/p300 interacting coactivator with glutamic acid/aspartic acid-rich tail 2 (CITED2) increased colon cancer cell invasiveness in vitro. Gene expression profiling revealed that CITED2 knockdown induced matrix metalloproteinase-13 (MMP-13) gene expression in colon cancer cells. Butyrate, a naturally occurring HDAC inhibitor, induced CITED2 expression and downregulated MMP-13 expression in RKO cells. Additionally, ectopic expression of CITED2 arrested RKO cell growth. Thus, CITED2 regulates colon cancer invasion and might be a target for HDAC inhibitor-based intervention of colon cancer.

Original language	English (US)
Pages (from-to)	5904-5910
Number of pages	7
Journal	FEBS Letters
Volume	581
Issue number	30
DOIs	https://doi.org/10.1016/j.febslet.2007.11.072
State	Published - Dec 22 2007
Externally published	Yes

Keywords

Butyrate
Histone deacetylase inhibitor
Matrix metalloproteinase-13
β-catenin

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2007.11.072

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title = "A role for CITED2, a CBP/p300 interacting protein, in colon cancer cell invasion",

abstract = "A thorough understanding of histone acetyltransferase CBP/p300-mediated regulation of gene expression and cell growth is essential to identify mechanisms relevant to the development of histone deacetylase (HDAC) inhibitor-based preventive and therapeutic strategies. We found that knockdown of CBP/p300 interacting coactivator with glutamic acid/aspartic acid-rich tail 2 (CITED2) increased colon cancer cell invasiveness in vitro. Gene expression profiling revealed that CITED2 knockdown induced matrix metalloproteinase-13 (MMP-13) gene expression in colon cancer cells. Butyrate, a naturally occurring HDAC inhibitor, induced CITED2 expression and downregulated MMP-13 expression in RKO cells. Additionally, ectopic expression of CITED2 arrested RKO cell growth. Thus, CITED2 regulates colon cancer invasion and might be a target for HDAC inhibitor-based intervention of colon cancer.",

keywords = "Butyrate, Histone deacetylase inhibitor, Matrix metalloproteinase-13, β-catenin",

author = "Longchuan Bai and Merchant, {Juanita L.}",

note = "Funding Information: Supported by NIH Grant R01-DK55732 (J.L.M.), Michigan Gastrointestinal Peptide Research Center Pilot Feasibility Grant P30-DK34933 (L.B.) and the John and Suzanne Munn Endowed Research Fund of the University of Michigan Cancer Center (L.B).",

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doi = "10.1016/j.febslet.2007.11.072",

language = "English (US)",

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T1 - A role for CITED2, a CBP/p300 interacting protein, in colon cancer cell invasion

AU - Bai, Longchuan

AU - Merchant, Juanita L.

N1 - Funding Information: Supported by NIH Grant R01-DK55732 (J.L.M.), Michigan Gastrointestinal Peptide Research Center Pilot Feasibility Grant P30-DK34933 (L.B.) and the John and Suzanne Munn Endowed Research Fund of the University of Michigan Cancer Center (L.B).

PY - 2007/12/22

Y1 - 2007/12/22

N2 - A thorough understanding of histone acetyltransferase CBP/p300-mediated regulation of gene expression and cell growth is essential to identify mechanisms relevant to the development of histone deacetylase (HDAC) inhibitor-based preventive and therapeutic strategies. We found that knockdown of CBP/p300 interacting coactivator with glutamic acid/aspartic acid-rich tail 2 (CITED2) increased colon cancer cell invasiveness in vitro. Gene expression profiling revealed that CITED2 knockdown induced matrix metalloproteinase-13 (MMP-13) gene expression in colon cancer cells. Butyrate, a naturally occurring HDAC inhibitor, induced CITED2 expression and downregulated MMP-13 expression in RKO cells. Additionally, ectopic expression of CITED2 arrested RKO cell growth. Thus, CITED2 regulates colon cancer invasion and might be a target for HDAC inhibitor-based intervention of colon cancer.

AB - A thorough understanding of histone acetyltransferase CBP/p300-mediated regulation of gene expression and cell growth is essential to identify mechanisms relevant to the development of histone deacetylase (HDAC) inhibitor-based preventive and therapeutic strategies. We found that knockdown of CBP/p300 interacting coactivator with glutamic acid/aspartic acid-rich tail 2 (CITED2) increased colon cancer cell invasiveness in vitro. Gene expression profiling revealed that CITED2 knockdown induced matrix metalloproteinase-13 (MMP-13) gene expression in colon cancer cells. Butyrate, a naturally occurring HDAC inhibitor, induced CITED2 expression and downregulated MMP-13 expression in RKO cells. Additionally, ectopic expression of CITED2 arrested RKO cell growth. Thus, CITED2 regulates colon cancer invasion and might be a target for HDAC inhibitor-based intervention of colon cancer.

KW - Butyrate

KW - Histone deacetylase inhibitor

KW - Matrix metalloproteinase-13

KW - β-catenin

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JO - FEBS Letters

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ER -

A role for CITED2, a CBP/p300 interacting protein, in colon cancer cell invasion

Abstract

Keywords

ASJC Scopus subject areas

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