A turn-off fluorescent substrate for horseradish peroxidase improves the sensitivity of ELISAs

Abhinav P. Acharya; Payel Sen; Kiana Aran; Austin B. Gardner; Mohammad Rafi; Deborah Dean; Niren Murthy

doi:10.1002/pola.27376

A turn-off fluorescent substrate for horseradish peroxidase improves the sensitivity of ELISAs

Abhinav P. Acharya
, Payel Sen
, Kiana Aran
, Austin B. Gardner
, Mohammad Rafi
, Deborah Dean
, Niren Murthy

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A new turn-off fluorescent substrate for horseradish peroxidase is introduced, termed clics, which enhances the sensitivity of commercial enzyme-linked immunosorbant assays (ELISA) by 32-fold and detects chlamydial infections in patients. Clics are cost-effective, stable, and can be easily incorporated in commercial ELISA kits, and therefore should have a great impact in the fields of medicine and biology.

Original language	English (US)
Pages (from-to)	206-210
Number of pages	5
Journal	Journal of Polymer Science, Part A: Polymer Chemistry
Volume	53
Issue number	2
DOIs	https://doi.org/10.1002/pola.27376
State	Published - Jan 15 2015
Externally published	Yes

Keywords

ELISA
biomaterials
chlamydia
cyanine
diagnostics
enzymes
liposome
nanotechnology
ultrasensitive

ASJC Scopus subject areas

Polymers and Plastics
Organic Chemistry
Materials Chemistry

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10.1002/pola.27376

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title = "A turn-off fluorescent substrate for horseradish peroxidase improves the sensitivity of ELISAs",

abstract = "A new turn-off fluorescent substrate for horseradish peroxidase is introduced, termed clics, which enhances the sensitivity of commercial enzyme-linked immunosorbant assays (ELISA) by 32-fold and detects chlamydial infections in patients. Clics are cost-effective, stable, and can be easily incorporated in commercial ELISA kits, and therefore should have a great impact in the fields of medicine and biology.",

keywords = "ELISA, biomaterials, chlamydia, cyanine, diagnostics, enzymes, liposome, nanotechnology, ultrasensitive",

author = "Acharya, \{Abhinav P.\} and Payel Sen and Kiana Aran and Gardner, \{Austin B.\} and Mohammad Rafi and Deborah Dean and Niren Murthy",

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doi = "10.1002/pola.27376",

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T1 - A turn-off fluorescent substrate for horseradish peroxidase improves the sensitivity of ELISAs

AU - Acharya, Abhinav P.

AU - Sen, Payel

AU - Aran, Kiana

AU - Gardner, Austin B.

AU - Rafi, Mohammad

AU - Dean, Deborah

AU - Murthy, Niren

PY - 2015/1/15

Y1 - 2015/1/15

N2 - A new turn-off fluorescent substrate for horseradish peroxidase is introduced, termed clics, which enhances the sensitivity of commercial enzyme-linked immunosorbant assays (ELISA) by 32-fold and detects chlamydial infections in patients. Clics are cost-effective, stable, and can be easily incorporated in commercial ELISA kits, and therefore should have a great impact in the fields of medicine and biology.

AB - A new turn-off fluorescent substrate for horseradish peroxidase is introduced, termed clics, which enhances the sensitivity of commercial enzyme-linked immunosorbant assays (ELISA) by 32-fold and detects chlamydial infections in patients. Clics are cost-effective, stable, and can be easily incorporated in commercial ELISA kits, and therefore should have a great impact in the fields of medicine and biology.

KW - ELISA

KW - biomaterials

KW - chlamydia

KW - cyanine

KW - diagnostics

KW - enzymes

KW - liposome

KW - nanotechnology

KW - ultrasensitive

UR - https://www.scopus.com/pages/publications/84915779244

UR - https://www.scopus.com/pages/publications/84915779244#tab=citedBy

U2 - 10.1002/pola.27376

DO - 10.1002/pola.27376

M3 - Article

SN - 0887-624X

VL - 53

SP - 206

EP - 210

JO - Journal of Polymer Science, Part A: Polymer Chemistry

JF - Journal of Polymer Science, Part A: Polymer Chemistry

IS - 2

ER -

A turn-off fluorescent substrate for horseradish peroxidase improves the sensitivity of ELISAs

Abstract

Keywords

ASJC Scopus subject areas

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