A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death

Jennifer L. Furman; Pui Wing Mok; Shengyi Shen; Cliff I. Stains; Indraneel Ghosh

doi:10.1039/c0cc02229b

A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death

Jennifer L. Furman, Pui Wing Mok, Shengyi Shen, Cliff I. Stains, Indraneel Ghosh

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.

Original language	English (US)
Pages (from-to)	397-399
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	1
DOIs	https://doi.org/10.1039/c0cc02229b
State	Published - Dec 6 2011

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Access to Document

10.1039/c0cc02229b

Cite this

@article{6082d9ac777b4545863c7a65be385a16,

title = "A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death",

abstract = "Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.",

author = "Furman, {Jennifer L.} and Mok, {Pui Wing} and Shengyi Shen and Stains, {Cliff I.} and Indraneel Ghosh",

year = "2011",

month = dec,

day = "6",

doi = "10.1039/c0cc02229b",

language = "English (US)",

volume = "47",

pages = "397--399",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "1",

}

TY - JOUR

T1 - A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death

AU - Furman, Jennifer L.

AU - Mok, Pui Wing

AU - Shen, Shengyi

AU - Stains, Cliff I.

AU - Ghosh, Indraneel

PY - 2011/12/6

Y1 - 2011/12/6

N2 - Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.

AB - Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.

UR - http://www.scopus.com/inward/record.url?scp=78649842342&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649842342&partnerID=8YFLogxK

U2 - 10.1039/c0cc02229b

DO - 10.1039/c0cc02229b

M3 - Article

C2 - 20830433

SN - 1359-7345

VL - 47

SP - 397

EP - 399

JO - Chemical Communications

JF - Chemical Communications

IS - 1

ER -

A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this