TY - CHAP
T1 - Analysis of Host Responses to Neisseria gonorrhoeae Using a Human Three-Dimensional Endometrial Epithelial Cell Model
AU - Łaniewski, Paweł
AU - Herbst-Kralovetz, Melissa M.
N1 - Publisher Copyright: © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2019
Y1 - 2019
N2 - Neisseria gonorrhoeae infections have been associated with complications including chronic endometritis and pelvic inflammatory disease. Robust in vitro models of the female reproductive tract are urgently needed to better understand the biological mechanisms leading to these pathophysiological changes. Our human three-dimensional (3D) endometrial epithelial cell (EEC) model, which is generated using the HEC-1A cell line and rotating wall vessel (RWV) bioreactor technology, replicates several hallmarks of endometrial tissue in vivo. Studying the interactions of N. gonorrhoeae with the host using this newly characterized human 3D EEC model allows for the investigation of unique mechanisms of gonococcal pathogenesis in the upper female reproductive tract. In this chapter, we describe methodologies that can be used to investigate the interactions of N. gonorrhoeae with the human 3D endometrial epithelium. Protocols for generating the human 3D EEC model using the RWV technology and assessing the host response (including morphological/ultrastructural changes to the epithelial cells; cytokine/chemokine secretion or gene expression changes) following infection with N. gonorrhoeae are presented.
AB - Neisseria gonorrhoeae infections have been associated with complications including chronic endometritis and pelvic inflammatory disease. Robust in vitro models of the female reproductive tract are urgently needed to better understand the biological mechanisms leading to these pathophysiological changes. Our human three-dimensional (3D) endometrial epithelial cell (EEC) model, which is generated using the HEC-1A cell line and rotating wall vessel (RWV) bioreactor technology, replicates several hallmarks of endometrial tissue in vivo. Studying the interactions of N. gonorrhoeae with the host using this newly characterized human 3D EEC model allows for the investigation of unique mechanisms of gonococcal pathogenesis in the upper female reproductive tract. In this chapter, we describe methodologies that can be used to investigate the interactions of N. gonorrhoeae with the human 3D endometrial epithelium. Protocols for generating the human 3D EEC model using the RWV technology and assessing the host response (including morphological/ultrastructural changes to the epithelial cells; cytokine/chemokine secretion or gene expression changes) following infection with N. gonorrhoeae are presented.
KW - Female reproductive tract
KW - Host–pathogen interactions
KW - Human epithelial cells
KW - Innate immunity
KW - Neisseria gonorrhoeae
KW - Organotypic mucosal model
KW - Rotating wall vessel bioreactor
KW - Uterine infection
UR - http://www.scopus.com/inward/record.url?scp=85066240162&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85066240162&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-9496-0_20
DO - 10.1007/978-1-4939-9496-0_20
M3 - Chapter
C2 - 31119633
T3 - Methods in Molecular Biology
SP - 347
EP - 361
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -