Analysis of subcellular surface structure, function and dynamics

D. Anselmetti; N. Hansmeier; J. Kalinowski; J. Martini; T. Merkle; R. Palmisano; R. Ros; K. Schmied; A. Sischka; K. Toensing

doi:10.1007/s00216-006-0789-3

Analysis of subcellular surface structure, function and dynamics

D. Anselmetti, N. Hansmeier, J. Kalinowski, J. Martini, T. Merkle, R. Palmisano, R. Ros, K. Schmied, A. Sischka, K. Toensing

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Analytics of single biological cells allows quantitative investigation from a structural, functional and dynamical point of view and opens novel possibilities to an unamplified subcellular analysis. In this article, we report on three different experimental methods and their applications to single cellular systems with a subcellular sensitivity down to the single molecule level. First, the subcellular surface structure of living bacteria (Corynebacterium glutamicum) was investigated with atomic force microscopy (AFM) at the resolution of individual surface layer (S-layer) proteins; discrimination of bacterial strains that lack the expression of hexagonally packed surface layer proteins was possible. Second, quantitative measurement of individual recognition events of membrane-bound receptors on living B-cells was achieved in single cell manipulation and probing experiments with optical tweezers (OT) force spectroscopy. And third, intracellular dynamics of translocating photoactivatable GFP in plant protoplasts (Nicotiana tabacum BY-2) was quantitatively monitored by two-photon laser scanning microscopy (2PLSM).

Original language	English (US)
Pages (from-to)	83-89
Number of pages	7
Journal	Analytical and bioanalytical chemistry
Volume	387
Issue number	1
DOIs	https://doi.org/10.1007/s00216-006-0789-3
State	Published - Jan 2007
Externally published	Yes

Keywords

Atomic force microscopy
Optical tweezers
Two-photon laser scanning microscopy

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

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10.1007/s00216-006-0789-3

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title = "Analysis of subcellular surface structure, function and dynamics",

abstract = "Analytics of single biological cells allows quantitative investigation from a structural, functional and dynamical point of view and opens novel possibilities to an unamplified subcellular analysis. In this article, we report on three different experimental methods and their applications to single cellular systems with a subcellular sensitivity down to the single molecule level. First, the subcellular surface structure of living bacteria (Corynebacterium glutamicum) was investigated with atomic force microscopy (AFM) at the resolution of individual surface layer (S-layer) proteins; discrimination of bacterial strains that lack the expression of hexagonally packed surface layer proteins was possible. Second, quantitative measurement of individual recognition events of membrane-bound receptors on living B-cells was achieved in single cell manipulation and probing experiments with optical tweezers (OT) force spectroscopy. And third, intracellular dynamics of translocating photoactivatable GFP in plant protoplasts (Nicotiana tabacum BY-2) was quantitatively monitored by two-photon laser scanning microscopy (2PLSM).",

keywords = "Atomic force microscopy, Optical tweezers, Two-photon laser scanning microscopy",

author = "D. Anselmetti and N. Hansmeier and J. Kalinowski and J. Martini and T. Merkle and R. Palmisano and R. Ros and K. Schmied and A. Sischka and K. Toensing",

note = "Funding Information: Acknowledgements Financial support from the Deutsche Forschungs-gemeinschaft (DFG) within the Collaborative Research Project SFB 613-Physics of molecular recognition and single molecule processes in organic systems (D.A.), the Federal Ministry of Education and Research of Germany (BMBF) for the Research Project MEMO-Foundations of laser-based characterization of metabolism and morphology of biological tissue (D.A.) and from the DFG grants ME 1116/4–2 and BIZ 7/1–2 (T.M.) is gratefully acknowledged.",

year = "2007",

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doi = "10.1007/s00216-006-0789-3",

language = "English (US)",

volume = "387",

pages = "83--89",

journal = "Analytical and bioanalytical chemistry",

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AU - Anselmetti, D.

AU - Hansmeier, N.

AU - Kalinowski, J.

AU - Martini, J.

AU - Merkle, T.

AU - Palmisano, R.

AU - Ros, R.

AU - Schmied, K.

AU - Sischka, A.

AU - Toensing, K.

N1 - Funding Information: Acknowledgements Financial support from the Deutsche Forschungs-gemeinschaft (DFG) within the Collaborative Research Project SFB 613-Physics of molecular recognition and single molecule processes in organic systems (D.A.), the Federal Ministry of Education and Research of Germany (BMBF) for the Research Project MEMO-Foundations of laser-based characterization of metabolism and morphology of biological tissue (D.A.) and from the DFG grants ME 1116/4–2 and BIZ 7/1–2 (T.M.) is gratefully acknowledged.

PY - 2007/1

Y1 - 2007/1

N2 - Analytics of single biological cells allows quantitative investigation from a structural, functional and dynamical point of view and opens novel possibilities to an unamplified subcellular analysis. In this article, we report on three different experimental methods and their applications to single cellular systems with a subcellular sensitivity down to the single molecule level. First, the subcellular surface structure of living bacteria (Corynebacterium glutamicum) was investigated with atomic force microscopy (AFM) at the resolution of individual surface layer (S-layer) proteins; discrimination of bacterial strains that lack the expression of hexagonally packed surface layer proteins was possible. Second, quantitative measurement of individual recognition events of membrane-bound receptors on living B-cells was achieved in single cell manipulation and probing experiments with optical tweezers (OT) force spectroscopy. And third, intracellular dynamics of translocating photoactivatable GFP in plant protoplasts (Nicotiana tabacum BY-2) was quantitatively monitored by two-photon laser scanning microscopy (2PLSM).

AB - Analytics of single biological cells allows quantitative investigation from a structural, functional and dynamical point of view and opens novel possibilities to an unamplified subcellular analysis. In this article, we report on three different experimental methods and their applications to single cellular systems with a subcellular sensitivity down to the single molecule level. First, the subcellular surface structure of living bacteria (Corynebacterium glutamicum) was investigated with atomic force microscopy (AFM) at the resolution of individual surface layer (S-layer) proteins; discrimination of bacterial strains that lack the expression of hexagonally packed surface layer proteins was possible. Second, quantitative measurement of individual recognition events of membrane-bound receptors on living B-cells was achieved in single cell manipulation and probing experiments with optical tweezers (OT) force spectroscopy. And third, intracellular dynamics of translocating photoactivatable GFP in plant protoplasts (Nicotiana tabacum BY-2) was quantitatively monitored by two-photon laser scanning microscopy (2PLSM).

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KW - Two-photon laser scanning microscopy

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Analysis of subcellular surface structure, function and dynamics

Abstract

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