Antibodies to tumor necrosis factor: a component of B cell immune responses with a role in tumor/host interaction

José Alexandre M. Barbuto; William J. Grimes; Evan M. Hersh

doi:10.1007/BF01517233

Antibodies to tumor necrosis factor: a component of B cell immune responses with a role in tumor/host interaction

José Alexandre M. Barbuto, William J. Grimes, Evan M. Hersh

Cancer Center

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8 Scopus citations

Abstract

Infiltrating B lymphocytes are found within tumors, where their role and the antigens they recognize are poorly defined. After in vitro expansion of these cells, we were able to detect the production of antibodies to tumor necrosis factor α (TNF) in 13 of 17 human tumors studied. These antibodies were detected by both enzyme-linked immunosorbent assay and by neutralization. Anti-TNF antibodies were not produced by resting peripheral blood B cells of normal subjects. However, anti-TNF antibodies were produced by B cells obtained from healthy individuals, after either in vivo or in vitro antigenic stimulation. This suggests that anti-TNF antibody production may constitute part of the overall B cell response to antigens. The intratumoral production of anti-TNF antibody may play a role in tumor/host interactions.

Original language	English (US)
Pages (from-to)	31-36
Number of pages	6
Journal	Cancer Immunology Immunotherapy
Volume	40
Issue number	1
DOIs	https://doi.org/10.1007/BF01517233
State	Published - Jan 1995

Keywords

TNF
Tumor necrosis factor
Tumor-infiltrating lymphocytes B lymphocytes

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Oncology
Cancer Research

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10.1007/BF01517233

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title = "Antibodies to tumor necrosis factor: a component of B cell immune responses with a role in tumor/host interaction",

abstract = "Infiltrating B lymphocytes are found within tumors, where their role and the antigens they recognize are poorly defined. After in vitro expansion of these cells, we were able to detect the production of antibodies to tumor necrosis factor α (TNF) in 13 of 17 human tumors studied. These antibodies were detected by both enzyme-linked immunosorbent assay and by neutralization. Anti-TNF antibodies were not produced by resting peripheral blood B cells of normal subjects. However, anti-TNF antibodies were produced by B cells obtained from healthy individuals, after either in vivo or in vitro antigenic stimulation. This suggests that anti-TNF antibody production may constitute part of the overall B cell response to antigens. The intratumoral production of anti-TNF antibody may play a role in tumor/host interactions.",

keywords = "TNF, Tumor necrosis factor, Tumor-infiltrating lymphocytes B lymphocytes",

author = "Barbuto, {Jos{\'e} Alexandre M.} and Grimes, {William J.} and Hersh, {Evan M.}",

year = "1995",

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T1 - Antibodies to tumor necrosis factor

T2 - a component of B cell immune responses with a role in tumor/host interaction

AU - Barbuto, José Alexandre M.

AU - Grimes, William J.

AU - Hersh, Evan M.

PY - 1995/1

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N2 - Infiltrating B lymphocytes are found within tumors, where their role and the antigens they recognize are poorly defined. After in vitro expansion of these cells, we were able to detect the production of antibodies to tumor necrosis factor α (TNF) in 13 of 17 human tumors studied. These antibodies were detected by both enzyme-linked immunosorbent assay and by neutralization. Anti-TNF antibodies were not produced by resting peripheral blood B cells of normal subjects. However, anti-TNF antibodies were produced by B cells obtained from healthy individuals, after either in vivo or in vitro antigenic stimulation. This suggests that anti-TNF antibody production may constitute part of the overall B cell response to antigens. The intratumoral production of anti-TNF antibody may play a role in tumor/host interactions.

AB - Infiltrating B lymphocytes are found within tumors, where their role and the antigens they recognize are poorly defined. After in vitro expansion of these cells, we were able to detect the production of antibodies to tumor necrosis factor α (TNF) in 13 of 17 human tumors studied. These antibodies were detected by both enzyme-linked immunosorbent assay and by neutralization. Anti-TNF antibodies were not produced by resting peripheral blood B cells of normal subjects. However, anti-TNF antibodies were produced by B cells obtained from healthy individuals, after either in vivo or in vitro antigenic stimulation. This suggests that anti-TNF antibody production may constitute part of the overall B cell response to antigens. The intratumoral production of anti-TNF antibody may play a role in tumor/host interactions.

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Antibodies to tumor necrosis factor: a component of B cell immune responses with a role in tumor/host interaction

Abstract

Keywords

ASJC Scopus subject areas

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