Bisphenol A inhibits cultured mouse ovarian follicle growth partially via the aryl hydrocarbon receptor signaling pathway

Ayelet Ziv-Gal; Zelieann R. Craig; Wei Wang; Jodi A. Flaws

doi:10.1016/j.reprotox.2013.07.022

Bisphenol A inhibits cultured mouse ovarian follicle growth partially via the aryl hydrocarbon receptor signaling pathway

Ayelet Ziv-Gal, Zelieann R. Craig, Wei Wang, Jodi A. Flaws

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

Bisphenol A (BPA) is an endocrine disruptor that inhibits growth of mouse ovarian follicles and disrupts steroidogenesis at a dose of 438. μM. However, the effects of lower doses of BPA and its mechanism of action in ovarian follicles are unknown. We hypothesized that low doses of BPA inhibit follicular growth and decrease estradiol levels through the aryl hydrocarbon receptor (AHR) pathway. Antral follicles from wild-type and Ahr knock-out (AhrKO) mice were cultured for 96. h. Follicle diameters and estradiol levels then were compared in wild-type and AhrKO follicles ± BPA (0.004-438. μM). BPA inhibited follicle growth (110-438. μM) and decreased estradiol levels (43.8-438. μM) in wild-type and AhrKO follicles. However, at BPA 110. μM, inhibition of growth in AhrKO follicles was attenuated compared to wild-type follicles. These data suggest that BPA may inhibit follicle growth partially via the AHR pathway, whereas its effects on estradiol synthesis likely involve other mechanisms.

Original language	English (US)
Pages (from-to)	58-67
Number of pages	10
Journal	Reproductive Toxicology
Volume	42
DOIs	https://doi.org/10.1016/j.reprotox.2013.07.022
State	Published - Dec 2013

Keywords

AHR
Antral follicle
BPA
Estradiol
Low dose
Ovary

ASJC Scopus subject areas

Toxicology

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10.1016/j.reprotox.2013.07.022

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title = "Bisphenol A inhibits cultured mouse ovarian follicle growth partially via the aryl hydrocarbon receptor signaling pathway",

abstract = "Bisphenol A (BPA) is an endocrine disruptor that inhibits growth of mouse ovarian follicles and disrupts steroidogenesis at a dose of 438. μM. However, the effects of lower doses of BPA and its mechanism of action in ovarian follicles are unknown. We hypothesized that low doses of BPA inhibit follicular growth and decrease estradiol levels through the aryl hydrocarbon receptor (AHR) pathway. Antral follicles from wild-type and Ahr knock-out (AhrKO) mice were cultured for 96. h. Follicle diameters and estradiol levels then were compared in wild-type and AhrKO follicles ± BPA (0.004-438. μM). BPA inhibited follicle growth (110-438. μM) and decreased estradiol levels (43.8-438. μM) in wild-type and AhrKO follicles. However, at BPA 110. μM, inhibition of growth in AhrKO follicles was attenuated compared to wild-type follicles. These data suggest that BPA may inhibit follicle growth partially via the AHR pathway, whereas its effects on estradiol synthesis likely involve other mechanisms.",

keywords = "AHR, Antral follicle, BPA, Estradiol, Low dose, Ovary",

author = "Ayelet Ziv-Gal and Craig, {Zelieann R.} and Wei Wang and Flaws, {Jodi A.}",

note = "Funding Information: This work was supported by: National Institute on Environmental Health NIH grants R01ES019178 (J.A.F.), K99ES021467 (Z.R.C.), and T32ES007326 (W.W.), an Environmental Toxicology Fellowship (A.Z.G.), and the Billie A. Field Fellowship in Reproductive Biology (W.W.). The authors also wish to thank Dr. Liying Gao for her continuous support and technical assistance and all the other members of the Flaws{\textquoteright} lab for their assistance and constructive input.",

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doi = "10.1016/j.reprotox.2013.07.022",

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AU - Craig, Zelieann R.

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N1 - Funding Information: This work was supported by: National Institute on Environmental Health NIH grants R01ES019178 (J.A.F.), K99ES021467 (Z.R.C.), and T32ES007326 (W.W.), an Environmental Toxicology Fellowship (A.Z.G.), and the Billie A. Field Fellowship in Reproductive Biology (W.W.). The authors also wish to thank Dr. Liying Gao for her continuous support and technical assistance and all the other members of the Flaws’ lab for their assistance and constructive input.

PY - 2013/12

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N2 - Bisphenol A (BPA) is an endocrine disruptor that inhibits growth of mouse ovarian follicles and disrupts steroidogenesis at a dose of 438. μM. However, the effects of lower doses of BPA and its mechanism of action in ovarian follicles are unknown. We hypothesized that low doses of BPA inhibit follicular growth and decrease estradiol levels through the aryl hydrocarbon receptor (AHR) pathway. Antral follicles from wild-type and Ahr knock-out (AhrKO) mice were cultured for 96. h. Follicle diameters and estradiol levels then were compared in wild-type and AhrKO follicles ± BPA (0.004-438. μM). BPA inhibited follicle growth (110-438. μM) and decreased estradiol levels (43.8-438. μM) in wild-type and AhrKO follicles. However, at BPA 110. μM, inhibition of growth in AhrKO follicles was attenuated compared to wild-type follicles. These data suggest that BPA may inhibit follicle growth partially via the AHR pathway, whereas its effects on estradiol synthesis likely involve other mechanisms.

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Bisphenol A inhibits cultured mouse ovarian follicle growth partially via the aryl hydrocarbon receptor signaling pathway

Abstract

Keywords

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