@article{c2b651d7242c4fa59fbd04c76a010c75,
title = "Chromosome-scale genome assembly of the brown anole (Anolis sagrei), an emerging model species",
abstract = "Rapid technological improvements are democratizing access to high quality, chromosome-scale genome assemblies. No longer the domain of only the most highly studied model organisms, now non-traditional and emerging model species can be genome-enabled using a combination of sequencing technologies and assembly software. Consequently, old ideas built on sparse sampling across the tree of life have recently been amended in the face of genomic data drawn from a growing number of high-quality reference genomes. Arguably the most valuable are those long-studied species for which much is already known about their biology; what many term emerging model species. Here, we report a highly complete chromosome-scale genome assembly for the brown anole, Anolis sagrei – a lizard species widely studied across a variety of disciplines and for which a high-quality reference genome was long overdue. This assembly exceeds the vast majority of existing reptile and snake genomes in contiguity (N50 = 253.6 Mb) and annotation completeness. Through the analysis of this genome and population resequence data, we examine the history of repetitive element accumulation, identify the X chromosome, and propose a hypothesis for the evolutionary history of fusions between autosomes and the X that led to the sex chromosomes of A. sagrei.",
author = "Geneva, {Anthony J.} and Sungdae Park and Bock, {Dan G.} and {de Mello}, {Pietro L.H.} and Fatih Sarigol and Marc Tollis and Donihue, {Colin M.} and Reynolds, {R. Graham} and Nathalie Feiner and Rasys, {Ashley M.} and Lauderdale, {James D.} and Minchey, {Sergio G.} and Alcala, {Aaron J.} and Infante, {Carlos R.} and Kolbe, {Jason J.} and Dolph Schluter and Menke, {Douglas B.} and Losos, {Jonathan B.}",
note = "Funding Information: Research, collection, and export permissions were granted by the Bahamas Environment, Science and Technology Commission, Bahamas National Trust, and the Bahamas Ministry of Agriculture and Marine Resources. Thank you to Alexis Harrison, Sofia Prado-Irwin, Shea Lambert, Dan Scantlebury, and Shannan Yates for their assistance in the field. We are grateful to Cory Hahn, Matthew Gage, Analisa Shields-Estrada, Jeff Breeze, and the postdoctoral fellows, graduate, and undergraduate students of the Losos lab for assisting with animal care. Thank you to Tonia Schwartz for providing advanced access to the Sceloporus undulatus genome for completeness and contiguity comparisons. All animal care procedures were approved by Harvard Institutional Animal Care and Use Committee Protocol 26-11. This work was supported by the National Science Foundation under grants DEB-1927194 to JBL and AJG, IOS-1827647 to DBM, and DEB-1927156 to DBM. Additionally, DGB was supported by a Natural Sciences and Engineering Research Council of Canada (NSERC) Postdoctoral Fellowship, and a Banting Postdoctoral Fellowship. This project was also made possible, in part, through the support of a grant from the John Templeton Foundation and Human Frontiers grant RGP0030/2020 to JBL and DBM. The opinions expressed in this publication are those of the authors and do not necessarily reflect the views of the John Templeton Foundation. Funding Information: Research, collection, and export permissions were granted by the Bahamas Environment, Science and Technology Commission, Bahamas National Trust, and the Bahamas Ministry of Agriculture and Marine Resources. Thank you to Alexis Harrison, Sofia Prado-Irwin, Shea Lambert, Dan Scantlebury, and Shannan Yates for their assistance in the field. We are grateful to Cory Hahn, Matthew Gage, Analisa Shields-Estrada, Jeff Breeze, and the postdoctoral fellows, graduate, and undergraduate students of the Losos lab for assisting with animal care. Thank you to Tonia Schwartz for providing advanced access to the Sceloporus undulatus genome for completeness and contiguity comparisons. All animal care procedures were approved by Harvard Institutional Animal Care and Use Committee Protocol 26-11. This work was supported by the National Science Foundation under grants DEB-1927194 to JBL and AJG, IOS-1827647 to DBM, and DEB-1927156 to DBM. Additionally, DGB was supported by a Natural Sciences and Engineering Research Council of Canada (NSERC) Postdoctoral Fellowship, and a Banting Postdoctoral Fellowship. This project was also made possible, in part, through the support of a grant from the John Templeton Foundation and Human Frontiers grant RGP0030/2020 to JBL and DBM. The opinions expressed in this publication are those of the authors and do not necessarily reflect the views of the John Templeton Foundation. Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = dec,
doi = "https://doi.org/10.1038/s42003-022-04074-5",
language = "English (US)",
volume = "5",
journal = "Communications biology",
issn = "2399-3642",
publisher = "Springer Nature",
number = "1",
}