Co-treatment of mouse antral follicles with 17β-estradiol interferes with mono-2-ethylhexyl phthalate (MEHP)-induced atresia and altered apoptosis gene expression

Zelieann R. Craig; Jeffrey Singh; Rupesh K. Gupta; Jodi A. Flaws

doi:10.1016/j.reprotox.2014.01.002

Co-treatment of mouse antral follicles with 17β-estradiol interferes with mono-2-ethylhexyl phthalate (MEHP)-induced atresia and altered apoptosis gene expression

Zelieann R. Craig, Jeffrey Singh, Rupesh K. Gupta, Jodi A. Flaws

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Mono-2-ethyhexyl phthalate (MEHP) is a metabolite of a plasticizer found in many consumer products. MEHP inhibits mouse ovarian follicle growth by reducing 17β-estradiol (E₂) production. Yet, whether MEHP causes follicle death (atresia) is unclear. We hypothesized that MEHP causes atresia by altering apoptosis gene expression, and that E₂ co-treatment blocks these effects. Follicles were exposed to MEHP (0.36-36μM)±E₂ for 48-96h to determine the effect of MEHP±E₂ on atresia and gene expression. MEHP increased atresia, but this effect was blocked by co-treatment with E₂. MEHP increased the expression of the pro-apoptotic gene Aifm1, but decreased that of the pro-apoptotic gene Bok and the anti-apoptotic gene Bcl2l10. E₂ interfered with MEHP-induced changes in Aifm1 and Bcl2l10. Our findings suggest that decreased E₂ levels are required for MEHP-induced follicle atresia and that Aifm1, Bok, and Bcl2l10 are involved in this process.

Original language	English (US)
Pages (from-to)	45-51
Number of pages	7
Journal	Reproductive Toxicology
Volume	45
DOIs	https://doi.org/10.1016/j.reprotox.2014.01.002
State	Published - Jun 2014

Keywords

Apoptosis
Estradiol
Follicle
Ovary
Phthalate

ASJC Scopus subject areas

Toxicology

Access to Document

10.1016/j.reprotox.2014.01.002

Cite this

@article{a488a29c87254514955092b3c5172600,

title = "Co-treatment of mouse antral follicles with 17β-estradiol interferes with mono-2-ethylhexyl phthalate (MEHP)-induced atresia and altered apoptosis gene expression",

abstract = "Mono-2-ethyhexyl phthalate (MEHP) is a metabolite of a plasticizer found in many consumer products. MEHP inhibits mouse ovarian follicle growth by reducing 17β-estradiol (E2) production. Yet, whether MEHP causes follicle death (atresia) is unclear. We hypothesized that MEHP causes atresia by altering apoptosis gene expression, and that E2 co-treatment blocks these effects. Follicles were exposed to MEHP (0.36-36μM)±E2 for 48-96h to determine the effect of MEHP±E2 on atresia and gene expression. MEHP increased atresia, but this effect was blocked by co-treatment with E2. MEHP increased the expression of the pro-apoptotic gene Aifm1, but decreased that of the pro-apoptotic gene Bok and the anti-apoptotic gene Bcl2l10. E2 interfered with MEHP-induced changes in Aifm1 and Bcl2l10. Our findings suggest that decreased E2 levels are required for MEHP-induced follicle atresia and that Aifm1, Bok, and Bcl2l10 are involved in this process.",

keywords = "Apoptosis, Estradiol, Follicle, Ovary, Phthalate",

author = "Craig, {Zelieann R.} and Jeffrey Singh and Gupta, {Rupesh K.} and Flaws, {Jodi A.}",

note = "Funding Information: The authors thank Timothy DelValle and Dr. Liying Gao for technical help. This work was supported by National Institute on Environmental Health grants K99ES021467 (ZRC), R01ES019178 (JAF), and T32ES007326 (RKG).",

year = "2014",

month = jun,

doi = "10.1016/j.reprotox.2014.01.002",

language = "English (US)",

volume = "45",

pages = "45--51",

journal = "Reproductive Toxicology",

issn = "0890-6238",

publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Co-treatment of mouse antral follicles with 17β-estradiol interferes with mono-2-ethylhexyl phthalate (MEHP)-induced atresia and altered apoptosis gene expression

AU - Craig, Zelieann R.

AU - Singh, Jeffrey

AU - Gupta, Rupesh K.

AU - Flaws, Jodi A.

N1 - Funding Information: The authors thank Timothy DelValle and Dr. Liying Gao for technical help. This work was supported by National Institute on Environmental Health grants K99ES021467 (ZRC), R01ES019178 (JAF), and T32ES007326 (RKG).

PY - 2014/6

Y1 - 2014/6

N2 - Mono-2-ethyhexyl phthalate (MEHP) is a metabolite of a plasticizer found in many consumer products. MEHP inhibits mouse ovarian follicle growth by reducing 17β-estradiol (E2) production. Yet, whether MEHP causes follicle death (atresia) is unclear. We hypothesized that MEHP causes atresia by altering apoptosis gene expression, and that E2 co-treatment blocks these effects. Follicles were exposed to MEHP (0.36-36μM)±E2 for 48-96h to determine the effect of MEHP±E2 on atresia and gene expression. MEHP increased atresia, but this effect was blocked by co-treatment with E2. MEHP increased the expression of the pro-apoptotic gene Aifm1, but decreased that of the pro-apoptotic gene Bok and the anti-apoptotic gene Bcl2l10. E2 interfered with MEHP-induced changes in Aifm1 and Bcl2l10. Our findings suggest that decreased E2 levels are required for MEHP-induced follicle atresia and that Aifm1, Bok, and Bcl2l10 are involved in this process.

AB - Mono-2-ethyhexyl phthalate (MEHP) is a metabolite of a plasticizer found in many consumer products. MEHP inhibits mouse ovarian follicle growth by reducing 17β-estradiol (E2) production. Yet, whether MEHP causes follicle death (atresia) is unclear. We hypothesized that MEHP causes atresia by altering apoptosis gene expression, and that E2 co-treatment blocks these effects. Follicles were exposed to MEHP (0.36-36μM)±E2 for 48-96h to determine the effect of MEHP±E2 on atresia and gene expression. MEHP increased atresia, but this effect was blocked by co-treatment with E2. MEHP increased the expression of the pro-apoptotic gene Aifm1, but decreased that of the pro-apoptotic gene Bok and the anti-apoptotic gene Bcl2l10. E2 interfered with MEHP-induced changes in Aifm1 and Bcl2l10. Our findings suggest that decreased E2 levels are required for MEHP-induced follicle atresia and that Aifm1, Bok, and Bcl2l10 are involved in this process.

KW - Apoptosis

KW - Estradiol

KW - Follicle

KW - Ovary

KW - Phthalate

UR - http://www.scopus.com/inward/record.url?scp=84893494364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893494364&partnerID=8YFLogxK

U2 - 10.1016/j.reprotox.2014.01.002

DO - 10.1016/j.reprotox.2014.01.002

M3 - Article

C2 - 24412242

SN - 0890-6238

VL - 45

SP - 45

EP - 51

JO - Reproductive Toxicology

JF - Reproductive Toxicology

ER -

Co-treatment of mouse antral follicles with 17β-estradiol interferes with mono-2-ethylhexyl phthalate (MEHP)-induced atresia and altered apoptosis gene expression

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this