TY - GEN
T1 - Comparative study of optical fluorescent nanosensors ('PEBBLEs') and fiber optic microsensors for oxygen sensing
AU - Chen-Esterlit, Z.
AU - Peteu, S. F.
AU - Clark, H. A.
AU - McDonald, W.
AU - Kopelman, R.
PY - 1999
Y1 - 1999
N2 - In this paper we report the use of phase sensitive fluorometry to obtain preliminary results from opto-chemical fluorescent oxygen nanosensors. PEBBLE (Probe Encapsulated By Biologically Localized Embedding) sensors were fabricated by immobilizing tris(4,7-diphenyl-1,10-phenanthroline)Ru(II) chloride and tris(1,10-phenanthroline)Ru(II) chloride within a polyacrylamide matrix. PEBBLEs have diameters of 20-200 nm and exhibit excellent performance for dissolved oxygen detection. Their performance is compared with micrometer-sized (10-20 μm) optical fiber sensors and free dye in solution. Oxygen sensing ability of PEBBLEs was tested in the presence of other quenchers and compared with free dyes in solution. While PEBBLEs have been developed for minimally invasive intracellular chemical analysis, they show additional advantages, such as increased dynamic range, compared to microsensors, and an absence of interference (quenching) by heavy ions, in contrast to free dye solutions.
AB - In this paper we report the use of phase sensitive fluorometry to obtain preliminary results from opto-chemical fluorescent oxygen nanosensors. PEBBLE (Probe Encapsulated By Biologically Localized Embedding) sensors were fabricated by immobilizing tris(4,7-diphenyl-1,10-phenanthroline)Ru(II) chloride and tris(1,10-phenanthroline)Ru(II) chloride within a polyacrylamide matrix. PEBBLEs have diameters of 20-200 nm and exhibit excellent performance for dissolved oxygen detection. Their performance is compared with micrometer-sized (10-20 μm) optical fiber sensors and free dye in solution. Oxygen sensing ability of PEBBLEs was tested in the presence of other quenchers and compared with free dyes in solution. While PEBBLEs have been developed for minimally invasive intracellular chemical analysis, they show additional advantages, such as increased dynamic range, compared to microsensors, and an absence of interference (quenching) by heavy ions, in contrast to free dye solutions.
UR - https://www.scopus.com/pages/publications/0032642131
UR - https://www.scopus.com/pages/publications/0032642131#tab=citedBy
M3 - Conference contribution
SN - 0819430722
T3 - Proceedings of SPIE - The International Society for Optical Engineering
SP - 156
EP - 163
BT - Proceedings of SPIE - The International Society for Optical Engineering
PB - Society of Photo-Optical Instrumentation Engineers
T2 - Proceedings of the 1999 Advances in Fluorescence Sensing Technology
Y2 - 24 January 1999 through 27 January 1999
ER -