Confocal microscopy for the biological and material sciences: principle, applications, limitations

G. J. Brakenhoff; H. T. Voort; K. Visscher

Confocal microscopy for the biological and material sciences: principle, applications, limitations

G. J. Brakenhoff, H. T. Voort, K. Visscher

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In the confocal scanning light microscope a specific volume is sampled during the imaging process. The physical process is explained, together with how the size of the pinholes used affects the actual size of this volume. The thus produced 3-dimensional imaging is of high quality but subject to a number of limitations. A novel (bilateral scanning) arrangement is presented which may relieve some of these. Use is made in this approach of a double sided scanning mirror element and a charge coupled device (CCD) for image collection.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Publisher	Publ by Int Soc for Optical Engineering
Pages	121-127
Number of pages	7
ISBN (Print)	0819405299
State	Published - 1992
Event	International Conference on Scientific Optical Imaging - Georgetown, WA, USA Duration: Nov 28 1990 → Dec 1 1990

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	1439

Other

Other	International Conference on Scientific Optical Imaging
City	Georgetown, WA, USA
Period	11/28/90 → 12/1/90

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Cite this

Brakenhoff, G. J., Voort, H. T., & Visscher, K. (1992). Confocal microscopy for the biological and material sciences: principle, applications, limitations. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 121-127). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1439). Publ by Int Soc for Optical Engineering.

Confocal microscopy for the biological and material sciences: principle, applications, limitations. / Brakenhoff, G. J.; Voort, H. T.; Visscher, K.
Proceedings of SPIE - The International Society for Optical Engineering. Publ by Int Soc for Optical Engineering, 1992. p. 121-127 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1439).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Brakenhoff, GJ, Voort, HT & Visscher, K 1992, Confocal microscopy for the biological and material sciences: principle, applications, limitations. in Proceedings of SPIE - The International Society for Optical Engineering. Proceedings of SPIE - The International Society for Optical Engineering, vol. 1439, Publ by Int Soc for Optical Engineering, pp. 121-127, International Conference on Scientific Optical Imaging, Georgetown, WA, USA, 11/28/90.

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ER -

Confocal microscopy for the biological and material sciences: principle, applications, limitations

Abstract

Publication series

Other

ASJC Scopus subject areas

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Cite this