TY - JOUR
T1 - Context-dependent redesign of robust synthetic gene circuits
AU - Stone, Austin
AU - Youssef, Abdelrahaman
AU - Rijal, Sadikshya
AU - Zhang, Rong
AU - Tian, Xiao Jun
N1 - Publisher Copyright: © 2024 The Authors
PY - 2024
Y1 - 2024
N2 - Cells provide dynamic platforms for executing exogenous genetic programs in synthetic biology, resulting in highly context-dependent circuit performance. Recent years have seen an increasing interest in understanding the intricacies of circuit–host relationships, their influence on the synthetic bioengineering workflow, and in devising strategies to alleviate undesired effects. We provide an overview of how emerging circuit–host interactions, such as growth feedback and resource competition, impact both deterministic and stochastic circuit behaviors. We also emphasize control strategies for mitigating these unwanted effects. This review summarizes the latest advances and the current state of host-aware and resource-aware design of synthetic gene circuits.
AB - Cells provide dynamic platforms for executing exogenous genetic programs in synthetic biology, resulting in highly context-dependent circuit performance. Recent years have seen an increasing interest in understanding the intricacies of circuit–host relationships, their influence on the synthetic bioengineering workflow, and in devising strategies to alleviate undesired effects. We provide an overview of how emerging circuit–host interactions, such as growth feedback and resource competition, impact both deterministic and stochastic circuit behaviors. We also emphasize control strategies for mitigating these unwanted effects. This review summarizes the latest advances and the current state of host-aware and resource-aware design of synthetic gene circuits.
KW - circuit–host interactions
KW - control-embedded circuit design
KW - feedback context factors
KW - metabolic burden
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U2 - 10.1016/j.tibtech.2024.01.003
DO - 10.1016/j.tibtech.2024.01.003
M3 - Review article
SN - 0167-7799
JO - Trends in Biotechnology
JF - Trends in Biotechnology
ER -