Context-dependent redesign of robust synthetic gene circuits

Austin Stone, Abdelrahaman Youssef, Sadikshya Rijal, Rong Zhang, Xiao Jun Tian

Research output: Contribution to journalReview articlepeer-review

3 Scopus citations

Abstract

Cells provide dynamic platforms for executing exogenous genetic programs in synthetic biology, resulting in highly context-dependent circuit performance. Recent years have seen an increasing interest in understanding the intricacies of circuit–host relationships, their influence on the synthetic bioengineering workflow, and in devising strategies to alleviate undesired effects. We provide an overview of how emerging circuit–host interactions, such as growth feedback and resource competition, impact both deterministic and stochastic circuit behaviors. We also emphasize control strategies for mitigating these unwanted effects. This review summarizes the latest advances and the current state of host-aware and resource-aware design of synthetic gene circuits.

Original languageEnglish (US)
JournalTrends in Biotechnology
DOIs
StateAccepted/In press - 2024

Keywords

  • circuit–host interactions
  • control-embedded circuit design
  • feedback context factors
  • metabolic burden

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

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