TY - JOUR
T1 - Conversion of high grade lymphoma tumor cell line to intermediate grade with tpa and bryostatin 1 as determined by polypeptide analysis on 2D gel electrophoresis
AU - Al-Katib, A.
AU - Mohammad, R. M.
AU - Mohamed, A. N.
AU - Pettit, George
AU - Sensenbrenner, L. L.
PY - 1990
Y1 - 1990
N2 - A high‐resolution two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE) was used for total cellular polypeptide mapping of two established lymphoma cell lines; MANCA, a Burkitt's line representing a high grade lymphoma (HGL) and WSU‐NHL (nodular histiocytic lymphoma) representing an intermediate grade lymphoma (IGL). Gels were digitized and analysed with an image scanning computer using the Elsie 4 system. Polypeptide mapping revealed striking similarities in 1100 polypeptide spots in both types. However, three polypeptides were unique to HGL (Molecular mass/isoelectric point (Mr/PI): 39/4·4, 35/5·6, 33/4·8), and one to IGL (95/4·7). In order to investigate the kinetics of expression of these polypeptides, the two cell lines were treated with two protein kinase C (PKC) activators, tumour promoting 12‐O tetradecanoylphorbol 13‐acetate (TPA) and bryostatin 1. 2D‐PAGE of the treated cells revealed that the HGL line loses its unique polypeptides and expresses a new one. The new polypeptide has the same Mr and PI as that unique to the untreated IGL (95/4·7). TPA or bryostatin 1 treatment of the IGL line for 72 h induced no significant changes. Our data show a unidirectional change from HGL to IGL, supporting the clinical notion that HGL is less differentiated than IGL. It also shows the similarity in the mode of action of bryostatin 1 and TPA in inducing these polypeptide changes.
AB - A high‐resolution two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE) was used for total cellular polypeptide mapping of two established lymphoma cell lines; MANCA, a Burkitt's line representing a high grade lymphoma (HGL) and WSU‐NHL (nodular histiocytic lymphoma) representing an intermediate grade lymphoma (IGL). Gels were digitized and analysed with an image scanning computer using the Elsie 4 system. Polypeptide mapping revealed striking similarities in 1100 polypeptide spots in both types. However, three polypeptides were unique to HGL (Molecular mass/isoelectric point (Mr/PI): 39/4·4, 35/5·6, 33/4·8), and one to IGL (95/4·7). In order to investigate the kinetics of expression of these polypeptides, the two cell lines were treated with two protein kinase C (PKC) activators, tumour promoting 12‐O tetradecanoylphorbol 13‐acetate (TPA) and bryostatin 1. 2D‐PAGE of the treated cells revealed that the HGL line loses its unique polypeptides and expresses a new one. The new polypeptide has the same Mr and PI as that unique to the untreated IGL (95/4·7). TPA or bryostatin 1 treatment of the IGL line for 72 h induced no significant changes. Our data show a unidirectional change from HGL to IGL, supporting the clinical notion that HGL is less differentiated than IGL. It also shows the similarity in the mode of action of bryostatin 1 and TPA in inducing these polypeptide changes.
KW - Differentiation
KW - Non‐Hodgkin's lymphoma
KW - Two‐dimensional protein gel electrophoresis
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U2 - 10.1002/hon.2900080203
DO - 10.1002/hon.2900080203
M3 - Article
C2 - 2344999
SN - 0278-0232
VL - 8
SP - 81
EP - 89
JO - Hematological Oncology
JF - Hematological Oncology
IS - 2
ER -