@article{49536e66a08c4332b787e7ba4db78e6a,
title = "DAG Lipase Activity Is Necessary for TRP Channel Regulation in Drosophila Photoreceptors",
abstract = "In Drosophila, a phospholipase C-mediated signaling cascade links photoexcitation of rhodopsin to the opening of the TRP/TRPL channels. A lipid product of the cascade, diacylglycerol (DAG) and its metabolite(s), polyunsaturated fatty acids (PUFAs), have both been proposed as potential excitatory messengers. A crucial enzyme in the understanding of this process is likely to be DAG lipase (DAGL). However, DAGLs that might fulfill this role have not been previously identified in any organism. In this work, the Drosophila DAGL gene, inaE, has been identified from mutants that are defective in photoreceptor responses to light. The inaE-encoded protein isoforms show high sequence similarity to known mammalian DAG lipases, exhibit DAG lipase activity in vitro, and are highly expressed in photoreceptors. Analyses of norpA inaE double mutants and severe inaE mutants show that normal DAGL activity is required for the generation of physiologically meaningful photoreceptor responses.",
keywords = "CELLBIO, SIGNALING, SYSNEURO",
author = "Leung, {Hung Tat} and Julie Tseng-Crank and Eunju Kim and Cecon Mahapatra and Shikoh Shino and Ying Zhou and Lingling An and Doerge, {Rebecca W.} and Pak, {William L.}",
note = "Funding Information: We thank Kim Gilbert for help with the manuscript preparation; Chaoxian Geng and Guohua Li for their contributions in the early phases of the work; and Eric Harness, Junko Kitamoto, and Chia-Ping Huang for their technical assistance. The inaE N125 mutant was a generous gift from Martin Heisenberg in the 70s. We gratefully acknowledge the receipt of Df(1)ben C02 , Df(1)AR10 , FM7c Kr-GFP , and Yp3 stocks from Howard Nash, John Thomas, Henry Chang, and Mary Bownes, respectively. Other stocks were obtained from the Bloomington Stock Center. The microarray work was carried out in the Center for Medical Genomics, Indiana University School of Medicine (Director: Howard Edenberg). Confocal microscopy was carried out in the departmental confocal facility (Director: Don Ready), the sorting of xl mutant embryos were carried out in Henry Chang's laboratory, electron microscopy was carried out in Purdue University Life Science Microscope Facility (Director: Debra Sherman), and lipase assays were performed in Purdue Metabolomics Profiling Facility. We gratefully acknowledge the help of Don Ready, Henry Chang, and Bruce Cooper. This work was supported by NEI grant EY00033 to W.L.P.",
year = "2008",
month = jun,
day = "26",
doi = "10.1016/j.neuron.2008.05.001",
language = "English (US)",
volume = "58",
pages = "884--896",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "6",
}