Delivery of plasmid DNA to mammalian cells using polymer-gold nanorod assemblies

James Ramos; Huang Chiao Huang; Kaushal Rege

doi:10.1007/978-1-62703-336-7_9

Delivery of plasmid DNA to mammalian cells using polymer-gold nanorod assemblies

James Ramos, Huang Chiao Huang, Kaushal Rege

Research output: Chapter in Book/Report/Conference proceeding › Chapter

4 Scopus citations

Abstract

Functionalized and surface-modified gold nanorods (GNRs) have emerged as promising vehicles for the delivery of several therapeutic agents. Ease of functionalization, increased stability, biocompatibility, and size-dependent plasmonic properties make gold nanorods attractive in sensing, imaging, and delivery to different cellular types. Here, we demonstrate the use of polyelectrolyte-coated GNRs (PE-GNRs) for delivering plasmid DNA to mammalian cells for transgene expression.

Original language	English (US)
Title of host publication	Cellular and Subcellular Nanotechnology
Subtitle of host publication	Methods and Protocols
Editors	Volkmar Weissig, Mark Olsen, Tamer Elbayoumi
Pages	81-91
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-62703-336-7_9
State	Published - 2013

Publication series

Name	Methods in Molecular Biology
Volume	991

Keywords

Cationic polymers
Gold nanoparticle
Nonviral gene delivery
Polyelectrolytes
Stability

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-62703-336-7_9

Cite this

Delivery of plasmid DNA to mammalian cells using polymer-gold nanorod assemblies. / Ramos, James; Huang, Huang Chiao; Rege, Kaushal.
Cellular and Subcellular Nanotechnology: Methods and Protocols. ed. / Volkmar Weissig; Mark Olsen; Tamer Elbayoumi. 2013. p. 81-91 (Methods in Molecular Biology; Vol. 991).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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AB - Functionalized and surface-modified gold nanorods (GNRs) have emerged as promising vehicles for the delivery of several therapeutic agents. Ease of functionalization, increased stability, biocompatibility, and size-dependent plasmonic properties make gold nanorods attractive in sensing, imaging, and delivery to different cellular types. Here, we demonstrate the use of polyelectrolyte-coated GNRs (PE-GNRs) for delivering plasmid DNA to mammalian cells for transgene expression.

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Delivery of plasmid DNA to mammalian cells using polymer-gold nanorod assemblies

Abstract

Publication series

Keywords

ASJC Scopus subject areas

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