TY - JOUR
T1 - Erratum
T2 - A transient reporter for editing enrichment (TREE) in human cells (Nucleic Acids Research(2019)47: 19(E120)Doi: 10.1093/nar/gkz713)
AU - Standage-Beier, Kylie
AU - Tekel, Stefan J.
AU - Brookhouser, Nicholas
AU - Schwarz, Grace
AU - Nguyen, Toan
AU - Wang, Xiao
AU - Brafman, David A.
N1 - Publisher Copyright: © The Author(s) 2020.
PY - 2021
Y1 - 2021
N2 - The reference to a recent article has been added to the Introduction: . . . Some progress has been made to help enrich for edited cells, such as co-transfecting plasmids with a fluorescent reporter and using flow cytometry to isolate reporter-positive cells. Similarly, base editors fused to fluorescent proteins have been used to enrich for edited cell populations (16,58). . . 58. Coelho M.A., Li S., Pane L.S., Firth M., Ciotta G., Wrigley J.D., Cuomo M.E., Maresca M., Taylor B.J.M. (2018) BE-FLARE: a fluorescent reporter of base editing activity reveals editing characteristics of APOBEC3A and APOBEC3B. BMC Biol. 16:150. This addition does not affect the results and conclusion of the study. May 2020: an additional correction was made to remove the sentence "However, these techniques are only reporters of transfection (RoT) and do not report on base editing activity within a cell population."from the introduction. This change has been made online but is not reflected in the print version of either the original article (https://doi.org/10.1093/nar/gkz713) or the corrigendum (https://doi.org/10.1093/nar/gkaa027).
AB - The reference to a recent article has been added to the Introduction: . . . Some progress has been made to help enrich for edited cells, such as co-transfecting plasmids with a fluorescent reporter and using flow cytometry to isolate reporter-positive cells. Similarly, base editors fused to fluorescent proteins have been used to enrich for edited cell populations (16,58). . . 58. Coelho M.A., Li S., Pane L.S., Firth M., Ciotta G., Wrigley J.D., Cuomo M.E., Maresca M., Taylor B.J.M. (2018) BE-FLARE: a fluorescent reporter of base editing activity reveals editing characteristics of APOBEC3A and APOBEC3B. BMC Biol. 16:150. This addition does not affect the results and conclusion of the study. May 2020: an additional correction was made to remove the sentence "However, these techniques are only reporters of transfection (RoT) and do not report on base editing activity within a cell population."from the introduction. This change has been made online but is not reflected in the print version of either the original article (https://doi.org/10.1093/nar/gkz713) or the corrigendum (https://doi.org/10.1093/nar/gkaa027).
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U2 - 10.1093/NAR/GKAA027
DO - 10.1093/NAR/GKAA027
M3 - Comment/debate
C2 - 31943066
SN - 0305-1048
VL - 48
SP - 1602
JO - Nucleic acids research
JF - Nucleic acids research
IS - 3
ER -