Establishment of hamster blastocyst-derived embryonic stem (ES) cells

Thomas Doetschman; Phillip Williams; Nobuyo Maeda

doi:10.1016/0012-1606(88)90204-7

Establishment of hamster blastocyst-derived embryonic stem (ES) cells

Thomas Doetschman, Phillip Williams, Nobuyo Maeda

Cellular and Molecular Medicine

Research output: Contribution to journal › Article › peer-review

180 Scopus citations

Abstract

The establishment of four ES cell lines from the Syrian "golden" hamster (Mesocricetus auratus) is described. The cells can be maintained in the undifferentiated state when grown on primary mouse embryonic fibroblast feeder layers. In suspension culture they spontaneously differentiate into embryoid bodies of increasing complexity which contain a variety of tissues including embryonic ectoderm and myocardium. All four lines-one female and three male-are karyotypically normal with 44 chromosomes. Hamster is the second species from which ES cells have been established. As in mouse, the cells should be useful for developmental and transgenic studies.

Original language	English (US)
Pages (from-to)	224-227
Number of pages	4
Journal	Developmental biology
Volume	127
Issue number	1
DOIs	https://doi.org/10.1016/0012-1606(88)90204-7
State	Published - May 1988

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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10.1016/0012-1606(88)90204-7

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title = "Establishment of hamster blastocyst-derived embryonic stem (ES) cells",

abstract = "The establishment of four ES cell lines from the Syrian {"}golden{"} hamster (Mesocricetus auratus) is described. The cells can be maintained in the undifferentiated state when grown on primary mouse embryonic fibroblast feeder layers. In suspension culture they spontaneously differentiate into embryoid bodies of increasing complexity which contain a variety of tissues including embryonic ectoderm and myocardium. All four lines-one female and three male-are karyotypically normal with 44 chromosomes. Hamster is the second species from which ES cells have been established. As in mouse, the cells should be useful for developmental and transgenic studies.",

author = "Thomas Doetschman and Phillip Williams and Nobuyo Maeda",

note = "Funding Information: Howles, and Beverly Koller (University of Wisconsin) for assistance with various aspects of this work, Oliver Smithies (University of Wisconsin) for his encouragement and support, and William Dove and Neal First (University of Wisconsin) for reviewing the manuscript. This work was supported by an NIH grant (GM20069) to Oliver Smithies. This Paper 2959 from the Laboratory of Genetics, University of Wisconsin, Madison.",

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doi = "10.1016/0012-1606(88)90204-7",

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T1 - Establishment of hamster blastocyst-derived embryonic stem (ES) cells

AU - Doetschman, Thomas

AU - Williams, Phillip

AU - Maeda, Nobuyo

N1 - Funding Information: Howles, and Beverly Koller (University of Wisconsin) for assistance with various aspects of this work, Oliver Smithies (University of Wisconsin) for his encouragement and support, and William Dove and Neal First (University of Wisconsin) for reviewing the manuscript. This work was supported by an NIH grant (GM20069) to Oliver Smithies. This Paper 2959 from the Laboratory of Genetics, University of Wisconsin, Madison.

PY - 1988/5

Y1 - 1988/5

N2 - The establishment of four ES cell lines from the Syrian "golden" hamster (Mesocricetus auratus) is described. The cells can be maintained in the undifferentiated state when grown on primary mouse embryonic fibroblast feeder layers. In suspension culture they spontaneously differentiate into embryoid bodies of increasing complexity which contain a variety of tissues including embryonic ectoderm and myocardium. All four lines-one female and three male-are karyotypically normal with 44 chromosomes. Hamster is the second species from which ES cells have been established. As in mouse, the cells should be useful for developmental and transgenic studies.

AB - The establishment of four ES cell lines from the Syrian "golden" hamster (Mesocricetus auratus) is described. The cells can be maintained in the undifferentiated state when grown on primary mouse embryonic fibroblast feeder layers. In suspension culture they spontaneously differentiate into embryoid bodies of increasing complexity which contain a variety of tissues including embryonic ectoderm and myocardium. All four lines-one female and three male-are karyotypically normal with 44 chromosomes. Hamster is the second species from which ES cells have been established. As in mouse, the cells should be useful for developmental and transgenic studies.

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Establishment of hamster blastocyst-derived embryonic stem (ES) cells

Abstract

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