Abstract
Aim: To observe the stability of BCG-induced insulin resistance model. Methods: The glucose tolerance, serum glucose, FFA, insulin, triglycerides, cholesterol, TNF-α and ALT level were measured. The change of GDR was measured by euglycemic clamp in model rats after given iv BCG 2, 4 and 8 weeks. Results: After 2, 4 and 8 weeks, the GIR and glucose tolerance of the animals deceased significantly. After 2, 4 and 8 weeks, BCG infusion resulted in a pronounced reduction in glucose tolerance and insulin-stimulated glucose disposal rate [GDR = GDR: (29 ± 6) vs (13 ± 7) mg·kg-1 ·min-1 2 weeks; (29 ± 6) vs (11 ± 7) mg·kg-1 ·min-1 4 weeks and (23 ± 3) vs (16 ± 3) mg·kg-1 ·min-1 8 weeks, respectively, P < 0.01]. BCG infusion resulted in a pronounced increase in the weights of the liver [(6.2 ± 0.9) vs (8.2 ± 1.3) g, P < 0.05] and spleens [(0.51 ± 0.11) vs (1.4 ± 0.4) g, P < 0.01]. The histo-pathological results showed that BCG infusion resulted severe inflammation in the livers and spleens and the ratio of β/α in pancreas increased. The serum levels of triglyceride, FFA and glucose were unchanged, but the level of serum TNF-α [(543 ± 60) vs (759 ± 137) pg·mL-1, P < 0.05] and insulin [(31 ± 5) vs (36 ± 5) mu·L-1, P > 0.05] increased. Conclusion: This novel model of immune insulin resistance is completely and constantly established.
Original language | English (US) |
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Pages (from-to) | 321-325 |
Number of pages | 5 |
Journal | Yaoxue Xuebao |
Volume | 37 |
Issue number | 5 |
State | Published - May 2002 |
Externally published | Yes |
Keywords
- BCG
- Diabetes
- Euglycemic clamp
- Insulin resistance
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)