Evaluating backscattering polarized light imaging microstructural mapping capabilities through neural tissue and analogous phantom imaging

Justina Bonaventura, Kellys Morara, Rhea Carlson, Courtney Comrie, Anne Leigh Twer, Elizabeth Hutchinson, Travis W. Sawyer

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Significance: Knowledge of fiber microstructure and orientation in the brain is critical for many applications. Polarized light imaging (PLI) has been shown to have potential for better understanding neural fiber microstructure and directionality due to the anisotropy in myelin sheaths surrounding nerve fibers of the brain. Continuing to advance backscattering based PLI systems could provide a valuable avenue for in vivo neural imaging. Aim: To assess the potential of backscattering PLI systems, the ability to resolve crossing fibers, and the sensitivity to fiber inclination and curvature are considered across different imaging wavelengths. Approach: Investigation of these areas of relative uncertainty is undergone through imaging potential phantoms alongside analogous regions of interest in fixed ferret brain samples with a five-wavelength backscattering Mueller matrix polarimeter. Results: Promising phantoms are discovered for which the retardance, diattenuation and depolarization mappings are derived from the Mueller matrix and studied to assess the sensitivity of this polarimeter configuration to fiber orientations and tissue structures. Conclusions: Rich avenues for future study include further classifying this polarimeter's sensitivity to fiber inclination and fiber direction to accurately produce microstructural maps of neural tissue.

Original languageEnglish (US)
Pages (from-to)52914
Number of pages1
JournalJournal of biomedical optics
Volume29
Issue number5
DOIs
StatePublished - May 1 2024

Keywords

  • Mueller matrix polarimetry
  • backscattering polarimetry
  • brain imaging
  • polarized light imaging
  • tissue phantoms

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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