Evaluation of drug carrier hepatotoxicity using primary cell culture models

Güneş Kibar; Subhadeep Dutta; Kaushal Rege; O. Berk Usta

doi:10.1016/j.nano.2023.102651

Evaluation of drug carrier hepatotoxicity using primary cell culture models

Güneş Kibar, Subhadeep Dutta, Kaushal Rege, O. Berk Usta

Engineering, Ira A. Fulton Schools of (IAFSE)

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

This study aims to establish a primary rat hepatocyte culture model to evaluate dose-dependent hepatotoxic effects of drug carriers (lipopolymer nanoparticles; LPNs) temporal. Primary rat hepatocyte cell cultures were used to determine half-maximal Inhibition Concentrations (IC₅₀) of the drug-carrier library. Drug-carrier library, at concentrations <50 μg/mL, is benign to primary rat hepatocytes as determined using albumin and urea secretions. Albumin, as a hepatic biomarker, exhibited a more sensitive and faster outcome, compared to urea, for the determination of the IC₅₀ value of LPNs. Temporal measurements of hepatic biomarkers including urea and albumin, and rigorous physicochemical (hydrodynamic diameter, surface charge, etc.) characterization, should be combined to evaluate the hepatotoxicity of drug carrier libraries in screens.

Original language	English (US)
Article number	102651
Journal	Nanomedicine: Nanotechnology, Biology, and Medicine
Volume	48
DOIs	https://doi.org/10.1016/j.nano.2023.102651
State	Published - Feb 2023

Keywords

Hepatotoxicity
In vitro culture
Lipopolymer nanoparticle (LPN)
Nanotoxicity
Primary rat hepatocyte

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Molecular Medicine
Biomedical Engineering
General Materials Science
Pharmaceutical Science

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10.1016/j.nano.2023.102651

Cite this

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title = "Evaluation of drug carrier hepatotoxicity using primary cell culture models",

abstract = "This study aims to establish a primary rat hepatocyte culture model to evaluate dose-dependent hepatotoxic effects of drug carriers (lipopolymer nanoparticles; LPNs) temporal. Primary rat hepatocyte cell cultures were used to determine half-maximal Inhibition Concentrations (IC50) of the drug-carrier library. Drug-carrier library, at concentrations <50 μg/mL, is benign to primary rat hepatocytes as determined using albumin and urea secretions. Albumin, as a hepatic biomarker, exhibited a more sensitive and faster outcome, compared to urea, for the determination of the IC50 value of LPNs. Temporal measurements of hepatic biomarkers including urea and albumin, and rigorous physicochemical (hydrodynamic diameter, surface charge, etc.) characterization, should be combined to evaluate the hepatotoxicity of drug carrier libraries in screens.",

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AU - Kibar, Güneş

AU - Dutta, Subhadeep

AU - Rege, Kaushal

AU - Usta, O. Berk

PY - 2023/2

Y1 - 2023/2

N2 - This study aims to establish a primary rat hepatocyte culture model to evaluate dose-dependent hepatotoxic effects of drug carriers (lipopolymer nanoparticles; LPNs) temporal. Primary rat hepatocyte cell cultures were used to determine half-maximal Inhibition Concentrations (IC50) of the drug-carrier library. Drug-carrier library, at concentrations <50 μg/mL, is benign to primary rat hepatocytes as determined using albumin and urea secretions. Albumin, as a hepatic biomarker, exhibited a more sensitive and faster outcome, compared to urea, for the determination of the IC50 value of LPNs. Temporal measurements of hepatic biomarkers including urea and albumin, and rigorous physicochemical (hydrodynamic diameter, surface charge, etc.) characterization, should be combined to evaluate the hepatotoxicity of drug carrier libraries in screens.

AB - This study aims to establish a primary rat hepatocyte culture model to evaluate dose-dependent hepatotoxic effects of drug carriers (lipopolymer nanoparticles; LPNs) temporal. Primary rat hepatocyte cell cultures were used to determine half-maximal Inhibition Concentrations (IC50) of the drug-carrier library. Drug-carrier library, at concentrations <50 μg/mL, is benign to primary rat hepatocytes as determined using albumin and urea secretions. Albumin, as a hepatic biomarker, exhibited a more sensitive and faster outcome, compared to urea, for the determination of the IC50 value of LPNs. Temporal measurements of hepatic biomarkers including urea and albumin, and rigorous physicochemical (hydrodynamic diameter, surface charge, etc.) characterization, should be combined to evaluate the hepatotoxicity of drug carrier libraries in screens.

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KW - Lipopolymer nanoparticle (LPN)

KW - Nanotoxicity

KW - Primary rat hepatocyte

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Evaluation of drug carrier hepatotoxicity using primary cell culture models

Abstract

Keywords

ASJC Scopus subject areas

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Findings on Drug Delivery Systems Reported by Investigators at Massachusetts General Hospital (Evaluation of Drug Carrier Hepatotoxicity Using Primary Cell Culture Models)

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Evaluation of drug carrier hepatotoxicity using primary cell culture models

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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Fingerprint

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Findings on Drug Delivery Systems Reported by Investigators at Massachusetts General Hospital (Evaluation of Drug Carrier Hepatotoxicity Using Primary Cell Culture Models)

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