TY - JOUR
T1 - Expression of J chain RNA in cell lines representing different stages of B lymphocyte differentiation
AU - Mather, Elizabeth L.
AU - Alt, Frederick W.
AU - Bothwell, Alfred L.M.
AU - Baltimore, David
AU - Koshland, Marian Elliott
N1 - Funding Information: We would like to thank Peter Schreier for providing access to the plasmacytoma cDNA library and Gordon Cann for the restriction mapping of the cloned J chain cDNA. This work was supported by grants from the American Cancer Society and the National Institutes of Health. F.A. is a special fellow of the Leukemia Society of America. A.L.M.B. was a postdoctoral fellow of the National Cancer Institute and the Medical Foundation. D.B. is a research professor of the American Cancer Society.
PY - 1981/2
Y1 - 1981/2
N2 - During B cell differentiation to pentamer IgM secretion, synthesis of the pentamer joining component, the J chain, is initiated. We investigated the mechanism for initiating J chain synthesis by analyzing murine cell lines representing different stages in B cell differentiation. The expression of functional J chain mRNA was evaluated by cell-free translation and specific immunoprecipitation of a J chain product. The expression of precursor mRNA was examined by hybridization with a J chain probe obtained by molecular cloning of cDNA. No J chain-specific RNA could be demonstrated in a lymphoma line representative of an undifferentiated B lymphocyte, but three species of J chain RNA were identified in hybrid cell lines representative of IgM-secreting plasma cells: a mature message of approximately 1.5 kb and two minor components of 2.5 and 0.92 kb. The encounter of a B cell with antigen or mitogen must therefore trigger events that effect either transcription of J chain sequences or their intranuclear stabilization.
AB - During B cell differentiation to pentamer IgM secretion, synthesis of the pentamer joining component, the J chain, is initiated. We investigated the mechanism for initiating J chain synthesis by analyzing murine cell lines representing different stages in B cell differentiation. The expression of functional J chain mRNA was evaluated by cell-free translation and specific immunoprecipitation of a J chain product. The expression of precursor mRNA was examined by hybridization with a J chain probe obtained by molecular cloning of cDNA. No J chain-specific RNA could be demonstrated in a lymphoma line representative of an undifferentiated B lymphocyte, but three species of J chain RNA were identified in hybrid cell lines representative of IgM-secreting plasma cells: a mature message of approximately 1.5 kb and two minor components of 2.5 and 0.92 kb. The encounter of a B cell with antigen or mitogen must therefore trigger events that effect either transcription of J chain sequences or their intranuclear stabilization.
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U2 - 10.1016/0092-8674(81)90132-X
DO - 10.1016/0092-8674(81)90132-X
M3 - Article
C2 - 6781757
SN - 0092-8674
VL - 23
SP - 369
EP - 378
JO - Cell
JF - Cell
IS - 2
ER -