Flow injection-chemiluminescent assay for the determination of superoxide dismutase activity

H. Y. Choi; J. H. Song; Y. S. Park; G. Lord; D. K. Park

doi:10.1139/cjc-79-3-337

Flow injection-chemiluminescent assay for the determination of superoxide dismutase activity

H. Y. Choi, J. H. Song, Y. S. Park, G. Lord, D. K. Park

Research output: Contribution to journal › Article › peer-review

Abstract

Superoxide dismutase (SOD) activity was measured using a flow injection-chemiluminescent assay (FI-CLA) based on xanthine-xanthine oxidase dependent superoxide (O₂^·-) formation. The mobile phase consists of 50 mM potassium phosphate buffer (pH 7.4) containing lucigenin (5 μM) and xanthine (0.3 mM). Under optimum conditions, bovine albumin did not affect chemiluminescence at concentrations of 1-1000 μg mL^-1 and KCN inhibited 100% of the Cu, Zn-SOD activity using 5 μL of a 0.23 mM concentration. The analysis of one sample was done in less than 30 s with a relative standard deviation of ±3.1%. SOD activity in the biological samples was correlated to the amount of exogenously applied SOD. The FI-CLA method reported here appears to be one of the faster and more useful tools used to assay SOD activity.

Original language	English (US)
Pages (from-to)	337-341
Number of pages	5
Journal	Canadian Journal of Chemistry
Volume	79
Issue number	3
DOIs	https://doi.org/10.1139/cjc-79-3-337
State	Published - 2001
Externally published	Yes

Keywords

Flow injection-chemiluminescent assay
Lucigenin
Superoxide
Superoxide dismutase
Xanthine oxidase

ASJC Scopus subject areas

Catalysis
General Chemistry
Organic Chemistry

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10.1139/cjc-79-3-337

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title = "Flow injection-chemiluminescent assay for the determination of superoxide dismutase activity",

abstract = "Superoxide dismutase (SOD) activity was measured using a flow injection-chemiluminescent assay (FI-CLA) based on xanthine-xanthine oxidase dependent superoxide (O2·-) formation. The mobile phase consists of 50 mM potassium phosphate buffer (pH 7.4) containing lucigenin (5 μM) and xanthine (0.3 mM). Under optimum conditions, bovine albumin did not affect chemiluminescence at concentrations of 1-1000 μg mL-1 and KCN inhibited 100% of the Cu, Zn-SOD activity using 5 μL of a 0.23 mM concentration. The analysis of one sample was done in less than 30 s with a relative standard deviation of ±3.1%. SOD activity in the biological samples was correlated to the amount of exogenously applied SOD. The FI-CLA method reported here appears to be one of the faster and more useful tools used to assay SOD activity.",

keywords = "Flow injection-chemiluminescent assay, Lucigenin, Superoxide, Superoxide dismutase, Xanthine oxidase",

author = "Choi, {H. Y.} and Song, {J. H.} and Park, {Y. S.} and G. Lord and Park, {D. K.}",

year = "2001",

doi = "10.1139/cjc-79-3-337",

language = "English (US)",

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journal = "Canadian Journal of Chemistry",

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T1 - Flow injection-chemiluminescent assay for the determination of superoxide dismutase activity

AU - Choi, H. Y.

AU - Song, J. H.

AU - Park, Y. S.

AU - Lord, G.

AU - Park, D. K.

PY - 2001

Y1 - 2001

N2 - Superoxide dismutase (SOD) activity was measured using a flow injection-chemiluminescent assay (FI-CLA) based on xanthine-xanthine oxidase dependent superoxide (O2·-) formation. The mobile phase consists of 50 mM potassium phosphate buffer (pH 7.4) containing lucigenin (5 μM) and xanthine (0.3 mM). Under optimum conditions, bovine albumin did not affect chemiluminescence at concentrations of 1-1000 μg mL-1 and KCN inhibited 100% of the Cu, Zn-SOD activity using 5 μL of a 0.23 mM concentration. The analysis of one sample was done in less than 30 s with a relative standard deviation of ±3.1%. SOD activity in the biological samples was correlated to the amount of exogenously applied SOD. The FI-CLA method reported here appears to be one of the faster and more useful tools used to assay SOD activity.

AB - Superoxide dismutase (SOD) activity was measured using a flow injection-chemiluminescent assay (FI-CLA) based on xanthine-xanthine oxidase dependent superoxide (O2·-) formation. The mobile phase consists of 50 mM potassium phosphate buffer (pH 7.4) containing lucigenin (5 μM) and xanthine (0.3 mM). Under optimum conditions, bovine albumin did not affect chemiluminescence at concentrations of 1-1000 μg mL-1 and KCN inhibited 100% of the Cu, Zn-SOD activity using 5 μL of a 0.23 mM concentration. The analysis of one sample was done in less than 30 s with a relative standard deviation of ±3.1%. SOD activity in the biological samples was correlated to the amount of exogenously applied SOD. The FI-CLA method reported here appears to be one of the faster and more useful tools used to assay SOD activity.

KW - Flow injection-chemiluminescent assay

KW - Lucigenin

KW - Superoxide

KW - Superoxide dismutase

KW - Xanthine oxidase

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DO - 10.1139/cjc-79-3-337

M3 - Article

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EP - 341

JO - Canadian Journal of Chemistry

JF - Canadian Journal of Chemistry

IS - 3

ER -

Flow injection-chemiluminescent assay for the determination of superoxide dismutase activity

Abstract

Keywords

ASJC Scopus subject areas

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