TY - JOUR
T1 - Genome-wide analysis of G-quadruplex in Spodoptera frugiperda
AU - Deng, Zhongyuan
AU - Ren, Yudong
AU - Guo, Lina
AU - Xie, Xingcheng
AU - Wang, Lixiang
AU - Li, Xianchun
N1 - Funding Information: National Science Foundation of China (NSFC)-Henan Joint major grant (No. U2004206 ); State Key Laboratory of Cotton Biology (No. CB2020A06 ); Horizontal joint project (No. 20220239A ) and the USDA National Institute of Food and Agriculture (hatch grant ARZT-1360890-H31-164 and multi-state grant ARZT-1370400-R31-172 (NC246)). Thanks for the suggestion of Sisi Liu doctor. The G-quadruplex discovery in Spodoptera frugiperda genome has been done on the supercomputing system in the National supercomputing Center in Zhengzhou. Publisher Copyright: © 2022 Elsevier B.V.
PY - 2023/1/31
Y1 - 2023/1/31
N2 - Spodoptera frugiperda (Lepidoptera: Noctuidae) is a globally distributed lepidopteran crop pest that has developed resistance to most insecticides. The G-quadruplex (G4) is a secondary structure in the genome enriched in the promoters for regulating gene expression. However, little is known about G4 in S. frugiperda, especially whether G4 is involved in insecticide resistance and pest control. In this study, 387,875 G4 motifs in the whole genome of S. frugiperda were identified by bioinformatics prediction. We found that 66.90 % of theseG4 structures were located in genic regions and highly enriched in the upstream regions of start codons. Functional and pathway analyses showed that the genes with G4 enriched in promoter regions participate in several metabolic processes. Further analyses showed that G4 structures occurred more frequently in the promoters of P450 and CarE gene families. It was also investigated that G4 ligand N-methyl mesoporphyrin IX (NMM) decreased P450 protein activity in larval midgut tissue. Cytotoxicity and bioassay results revealed that NMM and pesticides had synergistic effects on toxicity. In conclusion, our findings suggest that G4 motif could be a new potential target for pest control.
AB - Spodoptera frugiperda (Lepidoptera: Noctuidae) is a globally distributed lepidopteran crop pest that has developed resistance to most insecticides. The G-quadruplex (G4) is a secondary structure in the genome enriched in the promoters for regulating gene expression. However, little is known about G4 in S. frugiperda, especially whether G4 is involved in insecticide resistance and pest control. In this study, 387,875 G4 motifs in the whole genome of S. frugiperda were identified by bioinformatics prediction. We found that 66.90 % of theseG4 structures were located in genic regions and highly enriched in the upstream regions of start codons. Functional and pathway analyses showed that the genes with G4 enriched in promoter regions participate in several metabolic processes. Further analyses showed that G4 structures occurred more frequently in the promoters of P450 and CarE gene families. It was also investigated that G4 ligand N-methyl mesoporphyrin IX (NMM) decreased P450 protein activity in larval midgut tissue. Cytotoxicity and bioassay results revealed that NMM and pesticides had synergistic effects on toxicity. In conclusion, our findings suggest that G4 motif could be a new potential target for pest control.
KW - DNA secondary structure
KW - G-quadruplex
KW - NMM
KW - Pest control
KW - Spodoptera frugiperda
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U2 - 10.1016/j.ijbiomac.2022.12.018
DO - 10.1016/j.ijbiomac.2022.12.018
M3 - Article
C2 - 36481335
SN - 0141-8130
VL - 226
SP - 840
EP - 852
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -