Genomic Characterization of Parengyodontium torokii sp. nov., a Biofilm-Forming Fungus Isolated from Mars 2020 Assembly Facility

Ceth W. Parker; Marcus de Melo Teixeira; Nitin K. Singh; Huzefa A. Raja; Kristof B. Cank; Giada Spigolon; Nicholas H. Oberlies; Bridget M. Barker; Jason E. Stajich; Christopher E. Mason; Kasthuri Venkateswaran

doi:10.3390/jof8010066

Genomic Characterization of Parengyodontium torokii sp. nov., a Biofilm-Forming Fungus Isolated from Mars 2020 Assembly Facility

Ceth W. Parker, Marcus de Melo Teixeira, Nitin K. Singh, Huzefa A. Raja, Kristof B. Cank, Giada Spigolon, Nicholas H. Oberlies, Bridget M. Barker, Jason E. Stajich, Christopher E. Mason, Kasthuri Venkateswaran

Biological Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A fungal strain (FJII-L10-SW-P1) was isolated from the Mars 2020 spacecraft assembly facility and exhibited biofilm formation on spacecraft-qualified Teflon surfaces. The reconstruction of a six-loci gene tree (ITS, LSU, SSU, RPB1 and RPB2, and TEF1) using multi-locus sequence typing (MLST) analyses of the strain FJII-L10-SW-P1 supported a close relationship to other known Parengyodontium album subclade 3 isolates while being phylogenetically distinct from subclade 1 strains. The zig-zag rachides morphology of the conidiogenous cells and spindle-shaped conidia were the distinct morphological characteristics of the P. album subclade 3 strains. The MLST data and morphological analysis supported the conclusion that the P. album subclade 3 strains could be classified as a new species of the genus Parengyodontium and placed in the family Cordycipitaceae. The name Parengyodontium torokii sp. nov. is proposed to accommodate the strain, with FJII-L10-SW-P1 as the holotype. The genome of the FJII-L10-SW-P1 strain was sequenced, annotated, and the secondary metabolite clusters were identified. Genes predicted to be responsible for biofilm formation and adhesion to surfaces were identified. Homology-based assignment of gene ontologies to the predicted proteome of P. torokii revealed the presence of gene clusters responsible for synthesizing several metabolic compounds, including a cytochalasin that was also verified using traditional metabolomic analysis.

Original language	English (US)
Article number	66
Journal	Journal of Fungi
Volume	8
Issue number	1
DOIs	https://doi.org/10.3390/jof8010066
State	Published - Jan 2022

Keywords

Biofilm
Fungi
Genomics
Mars 2020 mission
Metabolomics
Morphological analysis
Phylogenetic analysis

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Plant Science
Microbiology (medical)

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10.3390/jof8010066

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Parker, C. W., Teixeira, M. D. M., Singh, N. K., Raja, H. A., Cank, K. B., Spigolon, G., Oberlies, N. H., Barker, B. M., Stajich, J. E., Mason, C. E., & Venkateswaran, K. (2022). Genomic Characterization of Parengyodontium torokii sp. nov., a Biofilm-Forming Fungus Isolated from Mars 2020 Assembly Facility. Journal of Fungi, 8(1), Article 66. https://doi.org/10.3390/jof8010066

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title = "Genomic Characterization of Parengyodontium torokii sp. nov., a Biofilm-Forming Fungus Isolated from Mars 2020 Assembly Facility",

abstract = "A fungal strain (FJII-L10-SW-P1) was isolated from the Mars 2020 spacecraft assembly facility and exhibited biofilm formation on spacecraft-qualified Teflon surfaces. The reconstruction of a six-loci gene tree (ITS, LSU, SSU, RPB1 and RPB2, and TEF1) using multi-locus sequence typing (MLST) analyses of the strain FJII-L10-SW-P1 supported a close relationship to other known Parengyodontium album subclade 3 isolates while being phylogenetically distinct from subclade 1 strains. The zig-zag rachides morphology of the conidiogenous cells and spindle-shaped conidia were the distinct morphological characteristics of the P. album subclade 3 strains. The MLST data and morphological analysis supported the conclusion that the P. album subclade 3 strains could be classified as a new species of the genus Parengyodontium and placed in the family Cordycipitaceae. The name Parengyodontium torokii sp. nov. is proposed to accommodate the strain, with FJII-L10-SW-P1 as the holotype. The genome of the FJII-L10-SW-P1 strain was sequenced, annotated, and the secondary metabolite clusters were identified. Genes predicted to be responsible for biofilm formation and adhesion to surfaces were identified. Homology-based assignment of gene ontologies to the predicted proteome of P. torokii revealed the presence of gene clusters responsible for synthesizing several metabolic compounds, including a cytochalasin that was also verified using traditional metabolomic analysis.",

keywords = "Biofilm, Fungi, Genomics, Mars 2020 mission, Metabolomics, Morphological analysis, Phylogenetic analysis",

author = "Parker, {Ceth W.} and Teixeira, {Marcus de Melo} and Singh, {Nitin K.} and Raja, {Huzefa A.} and Cank, {Kristof B.} and Giada Spigolon and Oberlies, {Nicholas H.} and Barker, {Bridget M.} and Stajich, {Jason E.} and Mason, {Christopher E.} and Kasthuri Venkateswaran",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2022",

month = jan,

doi = "10.3390/jof8010066",

language = "English (US)",

volume = "8",

journal = "Journal of Fungi",

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T1 - Genomic Characterization of Parengyodontium torokii sp. nov., a Biofilm-Forming Fungus Isolated from Mars 2020 Assembly Facility

AU - Parker, Ceth W.

AU - Teixeira, Marcus de Melo

AU - Singh, Nitin K.

AU - Raja, Huzefa A.

AU - Cank, Kristof B.

AU - Spigolon, Giada

AU - Oberlies, Nicholas H.

AU - Barker, Bridget M.

AU - Stajich, Jason E.

AU - Mason, Christopher E.

AU - Venkateswaran, Kasthuri

PY - 2022/1

Y1 - 2022/1

N2 - A fungal strain (FJII-L10-SW-P1) was isolated from the Mars 2020 spacecraft assembly facility and exhibited biofilm formation on spacecraft-qualified Teflon surfaces. The reconstruction of a six-loci gene tree (ITS, LSU, SSU, RPB1 and RPB2, and TEF1) using multi-locus sequence typing (MLST) analyses of the strain FJII-L10-SW-P1 supported a close relationship to other known Parengyodontium album subclade 3 isolates while being phylogenetically distinct from subclade 1 strains. The zig-zag rachides morphology of the conidiogenous cells and spindle-shaped conidia were the distinct morphological characteristics of the P. album subclade 3 strains. The MLST data and morphological analysis supported the conclusion that the P. album subclade 3 strains could be classified as a new species of the genus Parengyodontium and placed in the family Cordycipitaceae. The name Parengyodontium torokii sp. nov. is proposed to accommodate the strain, with FJII-L10-SW-P1 as the holotype. The genome of the FJII-L10-SW-P1 strain was sequenced, annotated, and the secondary metabolite clusters were identified. Genes predicted to be responsible for biofilm formation and adhesion to surfaces were identified. Homology-based assignment of gene ontologies to the predicted proteome of P. torokii revealed the presence of gene clusters responsible for synthesizing several metabolic compounds, including a cytochalasin that was also verified using traditional metabolomic analysis.

AB - A fungal strain (FJII-L10-SW-P1) was isolated from the Mars 2020 spacecraft assembly facility and exhibited biofilm formation on spacecraft-qualified Teflon surfaces. The reconstruction of a six-loci gene tree (ITS, LSU, SSU, RPB1 and RPB2, and TEF1) using multi-locus sequence typing (MLST) analyses of the strain FJII-L10-SW-P1 supported a close relationship to other known Parengyodontium album subclade 3 isolates while being phylogenetically distinct from subclade 1 strains. The zig-zag rachides morphology of the conidiogenous cells and spindle-shaped conidia were the distinct morphological characteristics of the P. album subclade 3 strains. The MLST data and morphological analysis supported the conclusion that the P. album subclade 3 strains could be classified as a new species of the genus Parengyodontium and placed in the family Cordycipitaceae. The name Parengyodontium torokii sp. nov. is proposed to accommodate the strain, with FJII-L10-SW-P1 as the holotype. The genome of the FJII-L10-SW-P1 strain was sequenced, annotated, and the secondary metabolite clusters were identified. Genes predicted to be responsible for biofilm formation and adhesion to surfaces were identified. Homology-based assignment of gene ontologies to the predicted proteome of P. torokii revealed the presence of gene clusters responsible for synthesizing several metabolic compounds, including a cytochalasin that was also verified using traditional metabolomic analysis.

KW - Biofilm

KW - Fungi

KW - Genomics

KW - Mars 2020 mission

KW - Metabolomics

KW - Morphological analysis

KW - Phylogenetic analysis

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U2 - 10.3390/jof8010066

DO - 10.3390/jof8010066

M3 - Article

SN - 2309-608X

VL - 8

JO - Journal of Fungi

JF - Journal of Fungi

IS - 1

M1 - 66

ER -

Genomic Characterization of Parengyodontium torokii sp. nov., a Biofilm-Forming Fungus Isolated from Mars 2020 Assembly Facility

Abstract

Keywords

ASJC Scopus subject areas

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