TY - JOUR
T1 - Genomic investigation of antimicrobial resistance in Brucella abortus strains isolated from cattle in Brazil
AU - Pereira, Carine Rodrigues
AU - Kato, Rodrigo Bentes
AU - Araújo, Fabrício Almeida
AU - da Silva, Alessandra Lima
AU - dos Santos, Roselane Gonçalves
AU - de Jesus Sousa, Thiago
AU - Neia, Raquel Costa
AU - da Silva, Saulo Britto
AU - Williamson, Charles H.D.
AU - Gillece, John
AU - Lage, Andrey Pereira
AU - O'Callaghan, David
AU - Pickard, Derek
AU - Ramos, Rommel Thiago Juca
AU - de Carvalho Azevedo, Vasco Ariston
AU - Foster, Jeffrey T.
AU - Dorneles, Elaine Maria Seles
N1 - Publisher Copyright: © 2023 Elsevier Inc.
PY - 2023/6
Y1 - 2023/6
N2 - The emergence of resistance to antimicrobials among Brucella spp. has highlighted the need to understand the genetic determinants responsible for these phenotypes. We investigated the gene targets associated with antimicrobial resistance in 53 genomes of B. abortus strains, isolated from cattle in Brazil, with resistance or intermediate susceptibility to antimicrobials. The genetic diversity of the genomes was evaluated using single-nucleotide polymorphism (SNP) differences among the isolates. We compared 18 genes (gyrB, bepD, bepE, norMII, norMI, parC, bepC, folP, gyrA, rpoB, folA, rsmG, marR, parE, mprF, oxyR, bepF, and bepG) previously described as related to antimicrobial resistance in Brucella spp. to those of the reference strain B. abortus 2308, which was susceptible to all tested antimicrobials. Eight genes had nonsynonymous mutations, deletions, or stop codons in at least one of the analyzed genomes. However, we did not observe any association between the genetic polymorphisms in the evaluated genes and the antimicrobial resistance phenotypes observed in the Brazilian analyzed strains (46 intermediate susceptible to rifampicin, 6 resistance to at least one antimicrobial and 1 multidrug resistance).
AB - The emergence of resistance to antimicrobials among Brucella spp. has highlighted the need to understand the genetic determinants responsible for these phenotypes. We investigated the gene targets associated with antimicrobial resistance in 53 genomes of B. abortus strains, isolated from cattle in Brazil, with resistance or intermediate susceptibility to antimicrobials. The genetic diversity of the genomes was evaluated using single-nucleotide polymorphism (SNP) differences among the isolates. We compared 18 genes (gyrB, bepD, bepE, norMII, norMI, parC, bepC, folP, gyrA, rpoB, folA, rsmG, marR, parE, mprF, oxyR, bepF, and bepG) previously described as related to antimicrobial resistance in Brucella spp. to those of the reference strain B. abortus 2308, which was susceptible to all tested antimicrobials. Eight genes had nonsynonymous mutations, deletions, or stop codons in at least one of the analyzed genomes. However, we did not observe any association between the genetic polymorphisms in the evaluated genes and the antimicrobial resistance phenotypes observed in the Brazilian analyzed strains (46 intermediate susceptible to rifampicin, 6 resistance to at least one antimicrobial and 1 multidrug resistance).
KW - Brucellosis
KW - Epidemiology
KW - Multidrug resistance
KW - Single-nucleotide polymorphism
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U2 - 10.1016/j.genrep.2023.101777
DO - 10.1016/j.genrep.2023.101777
M3 - Article
SN - 2452-0144
VL - 31
JO - Gene Reports
JF - Gene Reports
M1 - 101777
ER -