TY - JOUR
T1 - High-grade transgenic somatic chimeras from chicken embryonic stem cells
AU - Van De Lavoir, Marie Cecile
AU - Mather-Love, Christine
AU - Leighton, Philip
AU - Diamond, Jennifer H.
AU - Heyer, Babette S.
AU - Roberts, Rhys
AU - Zhu, Lei
AU - Winters-Digiacinto, Peggy
AU - Kerchner, Allyn
AU - Gessaro, Terri
AU - Swanberg, Susan
AU - Delany, Mary E.
AU - Etches, Robert J.
N1 - Funding Information: The authors thank Heather Meyers, Kathleen Beemer, David Deng and Mingxia Shi for their assistance in the laboratory and Dr J. Petitte for providing the pMIEM plasmid. This work was funded by the Small Business Innovation Research Program of the National Institutes of Child Health and Human Development grant number 2R44 HD39583-02 (MCL), the Small Business Innovation Research program of the US Department of Agriculture, grant number 2003-33610-13933 (CML), and the USDA National research Initiative Competitive Grants Program, grant number 02-35205-11568 (MED).
PY - 2006/1
Y1 - 2006/1
N2 - Male and female embryonic stem (ES) cell lines were derived from the area pellucidae of Stage X (EG&K) chicken embryos. These ES cell lines were grown in culture for extended periods of time and the majority of the cells retained a diploid karyotype. When reintroduced into Stage VI-X (EG&K) recipient embryos, the cES cells were able to contribute to all somatic tissues. By combining irradiation of the recipient embryo with exposure of the cES cells to the embryonic environment in diapause, a high frequency and extent of chimerism was obtained. High-grade chimeras, indistinguishable from the donor phenotype by feather pigmentation, were produced. A transgene encoding GFP was incorporated into the genome of cES cells under control of the ubiquitous promoter CX and GFP was widely expressed in somatic tissues. Although cES cells made extensive contributions to the somatic tissues, contribution to the germline was not observed.
AB - Male and female embryonic stem (ES) cell lines were derived from the area pellucidae of Stage X (EG&K) chicken embryos. These ES cell lines were grown in culture for extended periods of time and the majority of the cells retained a diploid karyotype. When reintroduced into Stage VI-X (EG&K) recipient embryos, the cES cells were able to contribute to all somatic tissues. By combining irradiation of the recipient embryo with exposure of the cES cells to the embryonic environment in diapause, a high frequency and extent of chimerism was obtained. High-grade chimeras, indistinguishable from the donor phenotype by feather pigmentation, were produced. A transgene encoding GFP was incorporated into the genome of cES cells under control of the ubiquitous promoter CX and GFP was widely expressed in somatic tissues. Although cES cells made extensive contributions to the somatic tissues, contribution to the germline was not observed.
KW - Avian
KW - Chicken
KW - Embryonic stem cells
KW - Transgenesis
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U2 - 10.1016/j.mod.2005.10.002
DO - 10.1016/j.mod.2005.10.002
M3 - Article
C2 - 16325380
SN - 0925-4773
VL - 123
SP - 31
EP - 41
JO - Mechanisms of Development
JF - Mechanisms of Development
IS - 1
ER -