Hydrophobic chalcogenide fibers for cell-based bio-optical sensors

Pierre Lucas; Mark R. Riley; Michelle A. Solis; Christophe Juncker; Jayne Collier; Dianne E. Boesewetter

doi:10.1117/12.610828

Hydrophobic chalcogenide fibers for cell-based bio-optical sensors

Pierre Lucas, Mark R. Riley, Michelle A. Solis, Christophe Juncker, Jayne Collier, Dianne E. Boesewetter

Research output: Contribution to journal › Conference article › peer-review

4 Scopus citations

Abstract

Chalcogenide fibers are shown to exhibit a hydrophobic surface behavior which results in detection enhancement for organic species in aqueous solutions. We use these fibers to monitor the infrared signature of human lung cells and detect the presence of toxic agents in the cell surrounding media. The signal is collected using a fiber evanescent wave spectroscopy set up with live human cells acting as a sensitizer for detection of minute quantities of toxicant. A monolayer of human alveolar epithelial cells form strong attachment at the surface of the fiber sensing zone and live in contact with the fiber while their IR spectra is collected remotely. Biochemical change in the living cells are detected during exposure to toxic agents. Variations in the spectroscopic features of the cells are observed in different spectral regions. Finally, the toxicity of Te₂As₃Se₅ fibers is investigated.

Original language	English (US)
Article number	28
Pages (from-to)	104-114
Number of pages	11
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	5691
DOIs	https://doi.org/10.1117/12.610828
State	Published - 2005
Event	Optical Fibers and Sensors for Medical Applications V - San Jose, CA, United States Duration: Jan 22 2005 → Jan 25 2005

Keywords

Bio-sensor
Chalcogenide glass fiber
Evanescent wave
Human lung cell
Hydrophobicity
IR spectroscopy

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

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10.1117/12.610828

Cite this

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title = "Hydrophobic chalcogenide fibers for cell-based bio-optical sensors",

abstract = "Chalcogenide fibers are shown to exhibit a hydrophobic surface behavior which results in detection enhancement for organic species in aqueous solutions. We use these fibers to monitor the infrared signature of human lung cells and detect the presence of toxic agents in the cell surrounding media. The signal is collected using a fiber evanescent wave spectroscopy set up with live human cells acting as a sensitizer for detection of minute quantities of toxicant. A monolayer of human alveolar epithelial cells form strong attachment at the surface of the fiber sensing zone and live in contact with the fiber while their IR spectra is collected remotely. Biochemical change in the living cells are detected during exposure to toxic agents. Variations in the spectroscopic features of the cells are observed in different spectral regions. Finally, the toxicity of Te2As3Se5 fibers is investigated.",

keywords = "Bio-sensor, Chalcogenide glass fiber, Evanescent wave, Human lung cell, Hydrophobicity, IR spectroscopy",

author = "Pierre Lucas and Riley, {Mark R.} and Solis, {Michelle A.} and Christophe Juncker and Jayne Collier and Boesewetter, {Dianne E.}",

year = "2005",

doi = "10.1117/12.610828",

language = "English (US)",

volume = "5691",

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journal = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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note = "Optical Fibers and Sensors for Medical Applications V ; Conference date: 22-01-2005 Through 25-01-2005",

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TY - JOUR

T1 - Hydrophobic chalcogenide fibers for cell-based bio-optical sensors

AU - Lucas, Pierre

AU - Riley, Mark R.

AU - Solis, Michelle A.

AU - Juncker, Christophe

AU - Collier, Jayne

AU - Boesewetter, Dianne E.

PY - 2005

Y1 - 2005

N2 - Chalcogenide fibers are shown to exhibit a hydrophobic surface behavior which results in detection enhancement for organic species in aqueous solutions. We use these fibers to monitor the infrared signature of human lung cells and detect the presence of toxic agents in the cell surrounding media. The signal is collected using a fiber evanescent wave spectroscopy set up with live human cells acting as a sensitizer for detection of minute quantities of toxicant. A monolayer of human alveolar epithelial cells form strong attachment at the surface of the fiber sensing zone and live in contact with the fiber while their IR spectra is collected remotely. Biochemical change in the living cells are detected during exposure to toxic agents. Variations in the spectroscopic features of the cells are observed in different spectral regions. Finally, the toxicity of Te2As3Se5 fibers is investigated.

AB - Chalcogenide fibers are shown to exhibit a hydrophobic surface behavior which results in detection enhancement for organic species in aqueous solutions. We use these fibers to monitor the infrared signature of human lung cells and detect the presence of toxic agents in the cell surrounding media. The signal is collected using a fiber evanescent wave spectroscopy set up with live human cells acting as a sensitizer for detection of minute quantities of toxicant. A monolayer of human alveolar epithelial cells form strong attachment at the surface of the fiber sensing zone and live in contact with the fiber while their IR spectra is collected remotely. Biochemical change in the living cells are detected during exposure to toxic agents. Variations in the spectroscopic features of the cells are observed in different spectral regions. Finally, the toxicity of Te2As3Se5 fibers is investigated.

KW - Bio-sensor

KW - Chalcogenide glass fiber

KW - Evanescent wave

KW - Human lung cell

KW - Hydrophobicity

KW - IR spectroscopy

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DO - 10.1117/12.610828

M3 - Conference article

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EP - 114

JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

M1 - 28

T2 - Optical Fibers and Sensors for Medical Applications V

Y2 - 22 January 2005 through 25 January 2005

ER -

Hydrophobic chalcogenide fibers for cell-based bio-optical sensors

Abstract

Keywords

ASJC Scopus subject areas

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