Identification of the Flavone-Inducible Counter-Defense Genes and Their cis-Elements in Helicoverpa armigera

Zhongyuan Deng; Yuting Zhang; Liying Fang; Min Zhang; Lixiang Wang; Xinzhi Ni; Xianchun Li

doi:10.3390/toxins15060365

Identification of the Flavone-Inducible Counter-Defense Genes and Their cis-Elements in Helicoverpa armigera

Zhongyuan Deng, Yuting Zhang, Liying Fang, Min Zhang, Lixiang Wang, Xinzhi Ni, Xianchun Li

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Flavone is widely found in plants and plays an important role in plant defense against pests. Many pests, such as Helicoverpa armigera, use flavone as a cue to upregulate counter-defense genes for detoxification of flavone. Yet the spectrum of the flavone-inducible genes and their linked cis-regulatory elements remains unclear. In this study, 48 differentially expressed genes (DEGs) were found by RNA-seq. These DEGs were mainly concentrated in the retinol metabolism and drug metabolism-cytochrome P450 pathways. Further in silico analysis of the promoter regions of 24 upregulated genes predicted two motifs through MEME and five previously characterized cis-elements including CRE, TRE, EcRE, XRE-AhR and ARE. Functional analysis of the two predicted motifs and two different versions of ARE (named ARE1 and ARE2) in the promoter region of the flavone-inducible carboxylesterase gene CCE001j verified that the two motifs and ARE2 are not responsible for flavone induction of H. armigera counter-defense genes, whereas ARE1 is a new xenobiotic response element to flavone (XRE-Fla) and plays a decisive role in flavone induction of CCE001j. This study is of great significance for further understanding the antagonistic interaction between plants and herbivorous insects.

Original language	English (US)
Article number	365
Journal	Toxins
Volume	15
Issue number	6
DOIs	https://doi.org/10.3390/toxins15060365
State	Published - Jun 2023

Keywords

ARE
Helicoverpa armigera
carboxylesterase
cis-transcriptional regulation
detoxification metabolism
flavone

ASJC Scopus subject areas

Toxicology
Health, Toxicology and Mutagenesis

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@article{a1cbd375d3304a7aa1bf8fdc45a120c0,

title = "Identification of the Flavone-Inducible Counter-Defense Genes and Their cis-Elements in Helicoverpa armigera",

abstract = "Flavone is widely found in plants and plays an important role in plant defense against pests. Many pests, such as Helicoverpa armigera, use flavone as a cue to upregulate counter-defense genes for detoxification of flavone. Yet the spectrum of the flavone-inducible genes and their linked cis-regulatory elements remains unclear. In this study, 48 differentially expressed genes (DEGs) were found by RNA-seq. These DEGs were mainly concentrated in the retinol metabolism and drug metabolism-cytochrome P450 pathways. Further in silico analysis of the promoter regions of 24 upregulated genes predicted two motifs through MEME and five previously characterized cis-elements including CRE, TRE, EcRE, XRE-AhR and ARE. Functional analysis of the two predicted motifs and two different versions of ARE (named ARE1 and ARE2) in the promoter region of the flavone-inducible carboxylesterase gene CCE001j verified that the two motifs and ARE2 are not responsible for flavone induction of H. armigera counter-defense genes, whereas ARE1 is a new xenobiotic response element to flavone (XRE-Fla) and plays a decisive role in flavone induction of CCE001j. This study is of great significance for further understanding the antagonistic interaction between plants and herbivorous insects.",

keywords = "ARE, Helicoverpa armigera, carboxylesterase, cis-transcriptional regulation, detoxification metabolism, flavone",

author = "Zhongyuan Deng and Yuting Zhang and Liying Fang and Min Zhang and Lixiang Wang and Xinzhi Ni and Xianchun Li",

note = "Funding Information: This research was funded by the USDA National Institute of Food and Agriculture (hatchgrant ARZT-1360890-H31-164 and multi-state grant ARZT-1370400-R31-172 (NC246)), the National Science Foundation of China (NSFC; Henan Joint major grant No. U200420044), Key Scientific Research Projects of Colleges and Universities in Henan Province (No. 21A210027), and the State Key Laboratory of Cotton Biology Open Fund (No. CB2020A06). Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = jun,

doi = "10.3390/toxins15060365",

language = "English (US)",

volume = "15",

journal = "Toxins",

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T1 - Identification of the Flavone-Inducible Counter-Defense Genes and Their cis-Elements in Helicoverpa armigera

AU - Deng, Zhongyuan

AU - Zhang, Yuting

AU - Fang, Liying

AU - Zhang, Min

AU - Wang, Lixiang

AU - Ni, Xinzhi

AU - Li, Xianchun

N1 - Funding Information: This research was funded by the USDA National Institute of Food and Agriculture (hatchgrant ARZT-1360890-H31-164 and multi-state grant ARZT-1370400-R31-172 (NC246)), the National Science Foundation of China (NSFC; Henan Joint major grant No. U200420044), Key Scientific Research Projects of Colleges and Universities in Henan Province (No. 21A210027), and the State Key Laboratory of Cotton Biology Open Fund (No. CB2020A06). Publisher Copyright: © 2023 by the authors.

PY - 2023/6

Y1 - 2023/6

N2 - Flavone is widely found in plants and plays an important role in plant defense against pests. Many pests, such as Helicoverpa armigera, use flavone as a cue to upregulate counter-defense genes for detoxification of flavone. Yet the spectrum of the flavone-inducible genes and their linked cis-regulatory elements remains unclear. In this study, 48 differentially expressed genes (DEGs) were found by RNA-seq. These DEGs were mainly concentrated in the retinol metabolism and drug metabolism-cytochrome P450 pathways. Further in silico analysis of the promoter regions of 24 upregulated genes predicted two motifs through MEME and five previously characterized cis-elements including CRE, TRE, EcRE, XRE-AhR and ARE. Functional analysis of the two predicted motifs and two different versions of ARE (named ARE1 and ARE2) in the promoter region of the flavone-inducible carboxylesterase gene CCE001j verified that the two motifs and ARE2 are not responsible for flavone induction of H. armigera counter-defense genes, whereas ARE1 is a new xenobiotic response element to flavone (XRE-Fla) and plays a decisive role in flavone induction of CCE001j. This study is of great significance for further understanding the antagonistic interaction between plants and herbivorous insects.

AB - Flavone is widely found in plants and plays an important role in plant defense against pests. Many pests, such as Helicoverpa armigera, use flavone as a cue to upregulate counter-defense genes for detoxification of flavone. Yet the spectrum of the flavone-inducible genes and their linked cis-regulatory elements remains unclear. In this study, 48 differentially expressed genes (DEGs) were found by RNA-seq. These DEGs were mainly concentrated in the retinol metabolism and drug metabolism-cytochrome P450 pathways. Further in silico analysis of the promoter regions of 24 upregulated genes predicted two motifs through MEME and five previously characterized cis-elements including CRE, TRE, EcRE, XRE-AhR and ARE. Functional analysis of the two predicted motifs and two different versions of ARE (named ARE1 and ARE2) in the promoter region of the flavone-inducible carboxylesterase gene CCE001j verified that the two motifs and ARE2 are not responsible for flavone induction of H. armigera counter-defense genes, whereas ARE1 is a new xenobiotic response element to flavone (XRE-Fla) and plays a decisive role in flavone induction of CCE001j. This study is of great significance for further understanding the antagonistic interaction between plants and herbivorous insects.

KW - ARE

KW - Helicoverpa armigera

KW - carboxylesterase

KW - cis-transcriptional regulation

KW - detoxification metabolism

KW - flavone

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DO - 10.3390/toxins15060365

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SN - 2072-6651

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JO - Toxins

JF - Toxins

IS - 6

M1 - 365

ER -

Identification of the Flavone-Inducible Counter-Defense Genes and Their cis-Elements in Helicoverpa armigera

Abstract

Keywords

ASJC Scopus subject areas

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