Improved isolation and crystallization of Photosystem I for structural analysis

Petra Fromme; Horst Tobias Witt

doi:10.1016/S0005-2728(98)00059-0

Improved isolation and crystallization of Photosystem I for structural analysis

Petra Fromme, Horst Tobias Witt

Research output: Contribution to journal › Article › peer-review

115 Scopus citations

Abstract

Stable trimeric Photosystem I (PS I) was isolated from the cyanobacterium Synechococcus elongatus by use of stereochemically pure β- dodecylmaltoside. Crystals of extremely pure PSI are grown by dialysis against low salt concentration. The improved PS I crystals were the basis for a PSI model derived from X-ray structure analysis at 4 resolution (Krauss et al., Nat. Struct. Biol. 3 (1996) 965-973; Schubert et al., J. Mol. Biol. 272 (1997) 741-769) and for detailed information on the electron transfer chain elaborated by techniques of magnetic resonance (H. Kass, Thesis, TU-Berlin, 1995; Kamlowski et al., Biochim. Biophys. Acta 1319 (1997) 188-198 and 199- 213; Bittl et al., Biochemistry 36 (1997) 12001-12004). Crystallization procedures using micro- and macroseeding are described as basis for the forthcoming structure analysis.

Original language	English (US)
Pages (from-to)	175-184
Number of pages	10
Journal	Biochimica et Biophysica Acta - Bioenergetics
Volume	1365
Issue number	1-2
DOIs	https://doi.org/10.1016/S0005-2728(98)00059-0
State	Published - Jun 10 1998
Externally published	Yes

Keywords

Crystallization
Membrane protein
Photosynthesis
Photosystem I
Structure analysis

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/S0005-2728(98)00059-0

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title = "Improved isolation and crystallization of Photosystem I for structural analysis",

abstract = "Stable trimeric Photosystem I (PS I) was isolated from the cyanobacterium Synechococcus elongatus by use of stereochemically pure β- dodecylmaltoside. Crystals of extremely pure PSI are grown by dialysis against low salt concentration. The improved PS I crystals were the basis for a PSI model derived from X-ray structure analysis at 4 resolution (Krauss et al., Nat. Struct. Biol. 3 (1996) 965-973; Schubert et al., J. Mol. Biol. 272 (1997) 741-769) and for detailed information on the electron transfer chain elaborated by techniques of magnetic resonance (H. Kass, Thesis, TU-Berlin, 1995; Kamlowski et al., Biochim. Biophys. Acta 1319 (1997) 188-198 and 199- 213; Bittl et al., Biochemistry 36 (1997) 12001-12004). Crystallization procedures using micro- and macroseeding are described as basis for the forthcoming structure analysis.",

keywords = "Crystallization, Membrane protein, Photosynthesis, Photosystem I, Structure analysis",

author = "Petra Fromme and Witt, {Horst Tobias}",

note = "Funding Information: We thankH . SchmidtD, . Difiore and C. Lfineberg for the excellentte chnicaal ssistanceO. ur thanksa re to P. Chitnis for providing specific antibodiesW. e want to thank all collaboratorso n the structural investigationfso r the fruitful cooperationa nd the permissionto use some of our publishedfi guresi n this article.W e thankH . K~iBa nd W. Lubitz for the disposalo f the model of P700 prior to publication. This work was supportedb y the DeutscheF or-schungsgemeinschSaoftn derforschungsbere3ic1h2 gerichteteM embranprozessaen d by the Fonds der chemischeInn dustrie.",

year = "1998",

month = jun,

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doi = "10.1016/S0005-2728(98)00059-0",

language = "English (US)",

volume = "1365",

pages = "175--184",

journal = "Biochimica et Biophysica Acta - Bioenergetics",

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T1 - Improved isolation and crystallization of Photosystem I for structural analysis

AU - Fromme, Petra

AU - Witt, Horst Tobias

N1 - Funding Information: We thankH . SchmidtD, . Difiore and C. Lfineberg for the excellentte chnicaal ssistanceO. ur thanksa re to P. Chitnis for providing specific antibodiesW. e want to thank all collaboratorso n the structural investigationfso r the fruitful cooperationa nd the permissionto use some of our publishedfi guresi n this article.W e thankH . K~iBa nd W. Lubitz for the disposalo f the model of P700 prior to publication. This work was supportedb y the DeutscheF or-schungsgemeinschSaoftn derforschungsbere3ic1h2 gerichteteM embranprozessaen d by the Fonds der chemischeInn dustrie.

PY - 1998/6/10

Y1 - 1998/6/10

N2 - Stable trimeric Photosystem I (PS I) was isolated from the cyanobacterium Synechococcus elongatus by use of stereochemically pure β- dodecylmaltoside. Crystals of extremely pure PSI are grown by dialysis against low salt concentration. The improved PS I crystals were the basis for a PSI model derived from X-ray structure analysis at 4 resolution (Krauss et al., Nat. Struct. Biol. 3 (1996) 965-973; Schubert et al., J. Mol. Biol. 272 (1997) 741-769) and for detailed information on the electron transfer chain elaborated by techniques of magnetic resonance (H. Kass, Thesis, TU-Berlin, 1995; Kamlowski et al., Biochim. Biophys. Acta 1319 (1997) 188-198 and 199- 213; Bittl et al., Biochemistry 36 (1997) 12001-12004). Crystallization procedures using micro- and macroseeding are described as basis for the forthcoming structure analysis.

AB - Stable trimeric Photosystem I (PS I) was isolated from the cyanobacterium Synechococcus elongatus by use of stereochemically pure β- dodecylmaltoside. Crystals of extremely pure PSI are grown by dialysis against low salt concentration. The improved PS I crystals were the basis for a PSI model derived from X-ray structure analysis at 4 resolution (Krauss et al., Nat. Struct. Biol. 3 (1996) 965-973; Schubert et al., J. Mol. Biol. 272 (1997) 741-769) and for detailed information on the electron transfer chain elaborated by techniques of magnetic resonance (H. Kass, Thesis, TU-Berlin, 1995; Kamlowski et al., Biochim. Biophys. Acta 1319 (1997) 188-198 and 199- 213; Bittl et al., Biochemistry 36 (1997) 12001-12004). Crystallization procedures using micro- and macroseeding are described as basis for the forthcoming structure analysis.

KW - Crystallization

KW - Membrane protein

KW - Photosynthesis

KW - Photosystem I

KW - Structure analysis

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JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

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ER -

Improved isolation and crystallization of Photosystem I for structural analysis

Abstract

Keywords

ASJC Scopus subject areas

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