In Situ Generated Novel 1H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells

Shuo Gao; Lei Zhao; Zhiqiang Fan; Vikram D. Kodibagkar; Li Liu; Hanqin Wang; Hong Xu; Mingli Tu; Bifu Hu; Chuanbin Cao; Zhenjian Zhang; Jian Xin Yu

doi:10.3389/fchem.2021.709581

In Situ Generated Novel ¹H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells

Shuo Gao, Lei Zhao, Zhiqiang Fan, Vikram D. Kodibagkar, Li Liu, Hanqin Wang, Hong Xu, Mingli Tu, Bifu Hu, Chuanbin Cao, Zhenjian Zhang, Jian Xin Yu

Engineering, Ira A. Fulton Schools of (IAFSE)

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

For wide applications of the lacZ gene in cellular/molecular biology, small animal investigations, and clinical assessments, the improvement of noninvasive imaging approaches to precisely assay gene expression has garnered much attention. In this study, we investigate a novel molecular platform in which alizarin 2-O-β-d-galactopyranoside AZ-1 acts as a lacZ gene/β-gal responsive ¹H-MRI probe to induce significant ¹H-MRI contrast changes in relaxation times T₁ and T₂ in situ as a concerted effect for the discovery of β-gal activity with the exposure of Fe³⁺. We also demonstrate the capability of this strategy for detecting β-gal activity with lacZ-transfected human MCF7 breast and PC3 prostate cancer cells by reaction-enhanced ¹H-MRI T₁ and T₂ relaxation mapping.

Original language	English (US)
Article number	709581
Journal	Frontiers in Chemistry
Volume	9
DOIs	https://doi.org/10.3389/fchem.2021.709581
State	Published - Jul 15 2021

Keywords

T and T relaxation mapping
in vitro H-MRI imaging
lacZ gene reporter
responsive Fe-based H-MRI agent
synthesis
β-galactosidase detection

ASJC Scopus subject areas

General Chemistry

Access to Document

10.3389/fchem.2021.709581

Cite this

@article{6957730283134b9996a052d7096f4924,

title = "In Situ Generated Novel 1H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells",

abstract = "For wide applications of the lacZ gene in cellular/molecular biology, small animal investigations, and clinical assessments, the improvement of noninvasive imaging approaches to precisely assay gene expression has garnered much attention. In this study, we investigate a novel molecular platform in which alizarin 2-O-β-d-galactopyranoside AZ-1 acts as a lacZ gene/β-gal responsive 1H-MRI probe to induce significant 1H-MRI contrast changes in relaxation times T1 and T2 in situ as a concerted effect for the discovery of β-gal activity with the exposure of Fe3+. We also demonstrate the capability of this strategy for detecting β-gal activity with lacZ-transfected human MCF7 breast and PC3 prostate cancer cells by reaction-enhanced 1H-MRI T1 and T2 relaxation mapping.",

keywords = "T and T relaxation mapping, in vitro H-MRI imaging, lacZ gene reporter, responsive Fe-based H-MRI agent, synthesis, β-galactosidase detection",

author = "Shuo Gao and Lei Zhao and Zhiqiang Fan and Kodibagkar, {Vikram D.} and Li Liu and Hanqin Wang and Hong Xu and Mingli Tu and Bifu Hu and Chuanbin Cao and Zhenjian Zhang and Yu, {Jian Xin}",

note = "Funding Information: This research was supported in part by the Research/Development Grants (2016QDJZR01 and HBMUPI201808) from the Biomedical Research Institute, fifth School of Medicine/Suizhou Central Hospital, Hubei University of Medicine, and the Natural Science Foundation of China (21877028). Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Gao, Zhao, Fan, Kodibagkar, Liu, Wang, Xu, Tu, Hu, Cao, Zhang and Yu.",

year = "2021",

month = jul,

day = "15",

doi = "10.3389/fchem.2021.709581",

language = "English (US)",

volume = "9",

journal = "Frontiers in Chemistry",

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publisher = "Frontiers Media S. A.",

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T1 - In Situ Generated Novel 1H MRI Reporter for β-Galactosidase Activity Detection and Visualization in Living Tumor Cells

AU - Gao, Shuo

AU - Zhao, Lei

AU - Fan, Zhiqiang

AU - Kodibagkar, Vikram D.

AU - Liu, Li

AU - Wang, Hanqin

AU - Xu, Hong

AU - Tu, Mingli

AU - Hu, Bifu

AU - Cao, Chuanbin

AU - Zhang, Zhenjian

AU - Yu, Jian Xin

N1 - Funding Information: This research was supported in part by the Research/Development Grants (2016QDJZR01 and HBMUPI201808) from the Biomedical Research Institute, fifth School of Medicine/Suizhou Central Hospital, Hubei University of Medicine, and the Natural Science Foundation of China (21877028). Publisher Copyright: © Copyright © 2021 Gao, Zhao, Fan, Kodibagkar, Liu, Wang, Xu, Tu, Hu, Cao, Zhang and Yu.

PY - 2021/7/15

Y1 - 2021/7/15

N2 - For wide applications of the lacZ gene in cellular/molecular biology, small animal investigations, and clinical assessments, the improvement of noninvasive imaging approaches to precisely assay gene expression has garnered much attention. In this study, we investigate a novel molecular platform in which alizarin 2-O-β-d-galactopyranoside AZ-1 acts as a lacZ gene/β-gal responsive 1H-MRI probe to induce significant 1H-MRI contrast changes in relaxation times T1 and T2 in situ as a concerted effect for the discovery of β-gal activity with the exposure of Fe3+. We also demonstrate the capability of this strategy for detecting β-gal activity with lacZ-transfected human MCF7 breast and PC3 prostate cancer cells by reaction-enhanced 1H-MRI T1 and T2 relaxation mapping.

AB - For wide applications of the lacZ gene in cellular/molecular biology, small animal investigations, and clinical assessments, the improvement of noninvasive imaging approaches to precisely assay gene expression has garnered much attention. In this study, we investigate a novel molecular platform in which alizarin 2-O-β-d-galactopyranoside AZ-1 acts as a lacZ gene/β-gal responsive 1H-MRI probe to induce significant 1H-MRI contrast changes in relaxation times T1 and T2 in situ as a concerted effect for the discovery of β-gal activity with the exposure of Fe3+. We also demonstrate the capability of this strategy for detecting β-gal activity with lacZ-transfected human MCF7 breast and PC3 prostate cancer cells by reaction-enhanced 1H-MRI T1 and T2 relaxation mapping.

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KW - synthesis

KW - β-galactosidase detection

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