Induced Neural Stem Cells Generated from Rat Fibroblasts

Guangjun Xi; Pingfang Hu; Cunye Qu; Shenfeng Qiu; Chang Tong; Qi Long Ying

doi:10.1016/j.gpb.2013.09.003

Induced Neural Stem Cells Generated from Rat Fibroblasts

Guangjun Xi, Pingfang Hu, Cunye Qu, Shenfeng Qiu, Chang Tong, Qi Long Ying

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The generation of induced tissue-specific stem cells has been hampered by the lack of well-established methods for the maintenance of pure tissue-specific stem cells like the ones we have for embryonic stem (ES) cell cultures. Using a cocktail of cytokines and small molecules, we demonstrate that primitive neural stem (NS) cells derived from mouse ES cells and rat embryos can be maintained. Furthermore, using the same set of cytokines and small molecules, we show that induced NS (iNS) cells can be generated from rat fibroblasts by forced expression of the transcriptional factors Oct4, Sox2 and c-Myc. The generation and long-term maintenance of iNS cells could have wide and momentous implications.

Original language	English (US)
Pages (from-to)	312-319
Number of pages	8
Journal	Genomics, Proteomics and Bioinformatics
Volume	11
Issue number	5
DOIs	https://doi.org/10.1016/j.gpb.2013.09.003
State	Published - Oct 2013

Keywords

Fibroblasts
Induced neural stem cells
Induced pluripotent stem cells
Rat
Stem cell self-renewal

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics
Computational Mathematics

Access to Document

10.1016/j.gpb.2013.09.003

Cite this

@article{92f018dd17ab4b1f87fecbc990290f33,

title = "Induced Neural Stem Cells Generated from Rat Fibroblasts",

abstract = "The generation of induced tissue-specific stem cells has been hampered by the lack of well-established methods for the maintenance of pure tissue-specific stem cells like the ones we have for embryonic stem (ES) cell cultures. Using a cocktail of cytokines and small molecules, we demonstrate that primitive neural stem (NS) cells derived from mouse ES cells and rat embryos can be maintained. Furthermore, using the same set of cytokines and small molecules, we show that induced NS (iNS) cells can be generated from rat fibroblasts by forced expression of the transcriptional factors Oct4, Sox2 and c-Myc. The generation and long-term maintenance of iNS cells could have wide and momentous implications.",

keywords = "Fibroblasts, Induced neural stem cells, Induced pluripotent stem cells, Rat, Stem cell self-renewal",

author = "Guangjun Xi and Pingfang Hu and Cunye Qu and Shenfeng Qiu and Chang Tong and Ying, {Qi Long}",

note = "Funding Information: We thank members of the Ying lab and the USC Stem Cell Core for technical assistance; Charles Ashton for critical reading of the manuscript. This work was supported by USC startup fund to QLY and in part by NIH (Grant No. R01OD010926) to QLY.",

year = "2013",

month = oct,

doi = "10.1016/j.gpb.2013.09.003",

language = "English (US)",

volume = "11",

pages = "312--319",

journal = "Genomics, Proteomics and Bioinformatics",

issn = "1672-0229",

publisher = "Beijing Genomics Institute",

number = "5",

}

TY - JOUR

T1 - Induced Neural Stem Cells Generated from Rat Fibroblasts

AU - Xi, Guangjun

AU - Hu, Pingfang

AU - Qu, Cunye

AU - Qiu, Shenfeng

AU - Tong, Chang

AU - Ying, Qi Long

N1 - Funding Information: We thank members of the Ying lab and the USC Stem Cell Core for technical assistance; Charles Ashton for critical reading of the manuscript. This work was supported by USC startup fund to QLY and in part by NIH (Grant No. R01OD010926) to QLY.

PY - 2013/10

Y1 - 2013/10

N2 - The generation of induced tissue-specific stem cells has been hampered by the lack of well-established methods for the maintenance of pure tissue-specific stem cells like the ones we have for embryonic stem (ES) cell cultures. Using a cocktail of cytokines and small molecules, we demonstrate that primitive neural stem (NS) cells derived from mouse ES cells and rat embryos can be maintained. Furthermore, using the same set of cytokines and small molecules, we show that induced NS (iNS) cells can be generated from rat fibroblasts by forced expression of the transcriptional factors Oct4, Sox2 and c-Myc. The generation and long-term maintenance of iNS cells could have wide and momentous implications.

AB - The generation of induced tissue-specific stem cells has been hampered by the lack of well-established methods for the maintenance of pure tissue-specific stem cells like the ones we have for embryonic stem (ES) cell cultures. Using a cocktail of cytokines and small molecules, we demonstrate that primitive neural stem (NS) cells derived from mouse ES cells and rat embryos can be maintained. Furthermore, using the same set of cytokines and small molecules, we show that induced NS (iNS) cells can be generated from rat fibroblasts by forced expression of the transcriptional factors Oct4, Sox2 and c-Myc. The generation and long-term maintenance of iNS cells could have wide and momentous implications.

KW - Fibroblasts

KW - Induced neural stem cells

KW - Induced pluripotent stem cells

KW - Rat

KW - Stem cell self-renewal

UR - http://www.scopus.com/inward/record.url?scp=84886395908&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84886395908&partnerID=8YFLogxK

U2 - 10.1016/j.gpb.2013.09.003

DO - 10.1016/j.gpb.2013.09.003

M3 - Article

C2 - 24076032

SN - 1672-0229

VL - 11

SP - 312

EP - 319

JO - Genomics, Proteomics and Bioinformatics

JF - Genomics, Proteomics and Bioinformatics

IS - 5

ER -

Induced Neural Stem Cells Generated from Rat Fibroblasts

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this