TY - JOUR
T1 - Innovative Rocuronium Bromide Topical Formulation for Targeted Skin Drug Delivery
T2 - Design, Comprehensive Characterization, In Vitro 2D/3D Human Cell Culture and Permeation
AU - Ruiz, Victor H.
AU - Encinas-Basurto, David
AU - Sun, Bo
AU - Eedara, Basanth Babu
AU - Roh, Eunmiri
AU - Alarcon, Neftali Ortega
AU - Curiel-Lewandrowski, Clara
AU - Bode, Ann M.
AU - Mansour, Heidi M.
N1 - Funding Information: All SEM images and data were collected in the W.M. Keck Center for Nano-Scale Imaging in the Department of Chemistry and Biochemistry at the University of Arizona, with funding from the W.M. Keck Foundation Grant. All FTIR spectra were collected in the W.M. Keck Center for Nano-Scale Imaging in the Department of Chemistry and Biochemistry at the University of Arizona. This instrument purchase was supported by Arizona Technology and Research Initiative Fund (A.R.S.§15-1648). The authors thank the Imaging Cores Materials Imaging and Characterization Facility supported by the University of Arizona Office of Research, Discovery and Innovation and the X-Ray Diffraction Facility of the Department of Chemistry and Biochemistry at The University of Arizona. The authors thank Jianqin Lu for access to the SpectraMax M3 multimode plate reader. The authors sincerely thank Brooke Beam-Masani and Andrei Astachkine for the core facility access and assistance. Funding Information: This work was supported by federal grant award NIH NCI Project Program Grant (PPG) P01CA229112. Publisher Copyright: © 2023 by the authors.
PY - 2023/5
Y1 - 2023/5
N2 - Cutaneous squamous cell carcinoma (cSCC) is the second-most common type of non-melanoma skin cancer and is linked to long-term exposure to ultraviolet (UV) radiation from the sun. Rocuronium bromide (RocBr) is an FDA-approved drug that targets p53-related protein kinase (PRPK) that inhibits the development of UV-induced cSCC. This study aimed to investigate the physicochemical properties and in vitro behavior of RocBr. Techniques such as thermal analysis, electron microscopy, spectroscopy and in vitro assays were used to characterize RocBr. A topical oil/water emulsion lotion formulation of RocBr was successfully developed and evaluated. The in vitro permeation behavior of RocBr from its lotion formulation was quantified with Strat-M® synthetic biomimetic membrane and EpiDerm™ 3D human skin tissue. Significant membrane retention of RocBr drug was evident and more retention was obtained with the lotion formulation compared with the solution. This is the first systematic and comprehensive study to report these findings.
AB - Cutaneous squamous cell carcinoma (cSCC) is the second-most common type of non-melanoma skin cancer and is linked to long-term exposure to ultraviolet (UV) radiation from the sun. Rocuronium bromide (RocBr) is an FDA-approved drug that targets p53-related protein kinase (PRPK) that inhibits the development of UV-induced cSCC. This study aimed to investigate the physicochemical properties and in vitro behavior of RocBr. Techniques such as thermal analysis, electron microscopy, spectroscopy and in vitro assays were used to characterize RocBr. A topical oil/water emulsion lotion formulation of RocBr was successfully developed and evaluated. The in vitro permeation behavior of RocBr from its lotion formulation was quantified with Strat-M® synthetic biomimetic membrane and EpiDerm™ 3D human skin tissue. Significant membrane retention of RocBr drug was evident and more retention was obtained with the lotion formulation compared with the solution. This is the first systematic and comprehensive study to report these findings.
KW - EpiDerm 3D human skin tissue
KW - Franz cell diffusion system
KW - HaCaT human skin cell line
KW - NHEK human primary skin cells
KW - Strat-M synthetic biomimetic membrane
KW - drug flux
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U2 - 10.3390/ijms24108776
DO - 10.3390/ijms24108776
M3 - Article
C2 - 37240122
SN - 1661-6596
VL - 24
JO - International journal of molecular sciences
JF - International journal of molecular sciences
IS - 10
M1 - 8776
ER -