TY - JOUR
T1 - Insulin binding to cells of several tissues of the early chick embryo
AU - Doetschman, Thomas C.
AU - Havaranis, Amalia S.
AU - Herrmann, Heinz
N1 - Funding Information: This investigation was supported by a grant (No. HD 03316) from the National Institute of Child Health and Human Development and by a grant from the Muscular Dystrophy Association of America. Participation of one of the authors (A.S.H.) was made possible through a grant from the University of Connecticut Research Foundation and from the Eli Lily Company. One of the authors (H.H.) is Maude K. Irving Research Professor of the American Cancer Society. The supply of eggs was de frayed from National Cancer Institute Grant No. CA 14733.
PY - 1975/11
Y1 - 1975/11
N2 - The binding of I125-labeled insulin to isolated cells from several tissues of the 3- and 4-day chick embryo was determined over a concentration range of insulin from 2 × 10-11 to 2 × 10-7 M. The cells were obtained from limb bud and nonlimb bud tissues of the 4-day chick, from the headless 3-day chick embryo, and from cartilage of the 12-day embryo. The amount of bound insulin was found to be similar for the cells from the different embryonic tissues. Some implications of these findings for the interpretation of the nature of the binding sites and the teratogenic effect of insulin are discussed.
AB - The binding of I125-labeled insulin to isolated cells from several tissues of the 3- and 4-day chick embryo was determined over a concentration range of insulin from 2 × 10-11 to 2 × 10-7 M. The cells were obtained from limb bud and nonlimb bud tissues of the 4-day chick, from the headless 3-day chick embryo, and from cartilage of the 12-day embryo. The amount of bound insulin was found to be similar for the cells from the different embryonic tissues. Some implications of these findings for the interpretation of the nature of the binding sites and the teratogenic effect of insulin are discussed.
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U2 - 10.1016/0012-1606(75)90277-8
DO - 10.1016/0012-1606(75)90277-8
M3 - Article
C2 - 1204932
SN - 0012-1606
VL - 47
SP - 228
EP - 232
JO - Developmental biology
JF - Developmental biology
IS - 1
ER -