Insulin growth factor 1 increases vascular smooth muscle nitric oxide production

R. Muniyappa; M. F. Walsh; J. S. Rangi; R. M. Zayas; P. R. Standley; J. L. Ram; J. R. Sowers

doi:10.1016/S0024-3205(97)00594-8

Insulin growth factor 1 increases vascular smooth muscle nitric oxide production

R. Muniyappa, M. F. Walsh, J. S. Rangi, R. M. Zayas, P. R. Standley, J. L. Ram, J. R. Sowers

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Abstract

Insulin like growth factor 1 (IGF-1) vasodilates, increases blood flow and lowers blood pressure; nitric oxide (NO) has been suggested to mediate some of these effects. We studied the role of IGF-1 in the regulation of NO production in vascular smooth muscle cells (VSMC). IGF-1 induced a concentration and time-dependent increase in NO release from endothelium-denuded aortic rings. Pre-incubation with cycloheximide or aminoguanidine blocked IGF-1-stimulated NO release. In addition, a six-fold increase in NO production by VSMC was seen upon incubation with IGF-1. These results suggest that IGF-1 induces NO release in VSMC through a process that involves new protein synthesis and the inducible isoform of nitric oxide synthase.

Original language	English (US)
Pages (from-to)	925-931
Number of pages	7
Journal	Life Sciences
Volume	61
Issue number	9
DOIs	https://doi.org/10.1016/S0024-3205(97)00594-8
State	Published - Jul 25 1997

Keywords

Aminoguanidine
IGF-1
Nitric oxide
Vascular smooth muscle cells

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/S0024-3205(97)00594-8

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title = "Insulin growth factor 1 increases vascular smooth muscle nitric oxide production",

abstract = "Insulin like growth factor 1 (IGF-1) vasodilates, increases blood flow and lowers blood pressure; nitric oxide (NO) has been suggested to mediate some of these effects. We studied the role of IGF-1 in the regulation of NO production in vascular smooth muscle cells (VSMC). IGF-1 induced a concentration and time-dependent increase in NO release from endothelium-denuded aortic rings. Pre-incubation with cycloheximide or aminoguanidine blocked IGF-1-stimulated NO release. In addition, a six-fold increase in NO production by VSMC was seen upon incubation with IGF-1. These results suggest that IGF-1 induces NO release in VSMC through a process that involves new protein synthesis and the inducible isoform of nitric oxide synthase.",

keywords = "Aminoguanidine, IGF-1, Nitric oxide, Vascular smooth muscle cells",

author = "R. Muniyappa and Walsh, {M. F.} and Rangi, {J. S.} and Zayas, {R. M.} and Standley, {P. R.} and Ram, {J. L.} and Sowers, {J. R.}",

note = "Funding Information: The authorsw ish to thank Drs. Ross Clark and Michael Cronin of Genentechf or the generous gift of hrIGF-1. This investigativew ork was supported,in part, by a Veterans Administration grant, an American Heart Association, Michigan Affiliate Grant and an NIH MH-47181 and GM-08167.",

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doi = "10.1016/S0024-3205(97)00594-8",

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T1 - Insulin growth factor 1 increases vascular smooth muscle nitric oxide production

AU - Muniyappa, R.

AU - Walsh, M. F.

AU - Rangi, J. S.

AU - Zayas, R. M.

AU - Standley, P. R.

AU - Ram, J. L.

AU - Sowers, J. R.

N1 - Funding Information: The authorsw ish to thank Drs. Ross Clark and Michael Cronin of Genentechf or the generous gift of hrIGF-1. This investigativew ork was supported,in part, by a Veterans Administration grant, an American Heart Association, Michigan Affiliate Grant and an NIH MH-47181 and GM-08167.

PY - 1997/7/25

Y1 - 1997/7/25

N2 - Insulin like growth factor 1 (IGF-1) vasodilates, increases blood flow and lowers blood pressure; nitric oxide (NO) has been suggested to mediate some of these effects. We studied the role of IGF-1 in the regulation of NO production in vascular smooth muscle cells (VSMC). IGF-1 induced a concentration and time-dependent increase in NO release from endothelium-denuded aortic rings. Pre-incubation with cycloheximide or aminoguanidine blocked IGF-1-stimulated NO release. In addition, a six-fold increase in NO production by VSMC was seen upon incubation with IGF-1. These results suggest that IGF-1 induces NO release in VSMC through a process that involves new protein synthesis and the inducible isoform of nitric oxide synthase.

AB - Insulin like growth factor 1 (IGF-1) vasodilates, increases blood flow and lowers blood pressure; nitric oxide (NO) has been suggested to mediate some of these effects. We studied the role of IGF-1 in the regulation of NO production in vascular smooth muscle cells (VSMC). IGF-1 induced a concentration and time-dependent increase in NO release from endothelium-denuded aortic rings. Pre-incubation with cycloheximide or aminoguanidine blocked IGF-1-stimulated NO release. In addition, a six-fold increase in NO production by VSMC was seen upon incubation with IGF-1. These results suggest that IGF-1 induces NO release in VSMC through a process that involves new protein synthesis and the inducible isoform of nitric oxide synthase.

KW - Aminoguanidine

KW - IGF-1

KW - Nitric oxide

KW - Vascular smooth muscle cells

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JO - Life Sciences

JF - Life Sciences

IS - 9

ER -

Insulin growth factor 1 increases vascular smooth muscle nitric oxide production

Abstract

Keywords

ASJC Scopus subject areas

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