TY - JOUR
T1 - Interleukin-36γIs Elevated in Cervicovaginal Epithelial Cells in Women with Bacterial Vaginosis and in Vitro after Infection with Microbes Associated with Bacterial Vaginosis
AU - Gardner, Jameson K.
AU - Łaniewski, Paweł
AU - Knight, Anna
AU - Haddad, Lisa B.
AU - Swaims-Kohlmeier, Alison
AU - Herbst-Kralovetz, Melissa M.
N1 - Funding Information: Financial support. This work was funded by the National Institutes of Health (K23HD078153-01A1 [to L. B. H.] and 1R15AI113457-01A1 [to M. M. H. K.]), Alternatives Research and Development Foundation (to P. L. and M. M. H.-K.), and Valley Research Partnership P12 (to J. G. and M. M. H.-K.). Potential conflicts of interest. All authors: No reported conflicts of interest. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Publisher Copyright: © 2019 The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].
PY - 2020/3/15
Y1 - 2020/3/15
N2 - In recent studies, the interleukin (IL)-36 cytokines were shown to be elevated in women with non-Lactobacillus-dominated vaginal microbiomes. In this study, we evaluated IL36G expression in clinical samples from women with and without bacterial vaginosis (BV) and a human 3-dimensional cervical epithelial cell model. IL36G expression was significantly elevated in cervicovaginal epithelial cells isolated from BV-positive women and corresponded with increased neutrophil counts relative to BV-negative women. In addition, specific BV-associated bacterial species as well as a polymicrobial cocktail significantly induced IL36G expression in vitro. These findings suggest that IL-36γmay exhibit an important function in the host response to BV and other sexually transmitted infections.
AB - In recent studies, the interleukin (IL)-36 cytokines were shown to be elevated in women with non-Lactobacillus-dominated vaginal microbiomes. In this study, we evaluated IL36G expression in clinical samples from women with and without bacterial vaginosis (BV) and a human 3-dimensional cervical epithelial cell model. IL36G expression was significantly elevated in cervicovaginal epithelial cells isolated from BV-positive women and corresponded with increased neutrophil counts relative to BV-negative women. In addition, specific BV-associated bacterial species as well as a polymicrobial cocktail significantly induced IL36G expression in vitro. These findings suggest that IL-36γmay exhibit an important function in the host response to BV and other sexually transmitted infections.
KW - Atopobium vaginae
KW - Gardnerella vaginalis
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U2 - 10.1093/infdis/jiz514
DO - 10.1093/infdis/jiz514
M3 - Article
C2 - 31586390
SN - 0022-1899
VL - 221
SP - 983
EP - 988
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 6
ER -