Isolation of megabase-size DNA from sorghum and applications for physical mapping and bacterial and yeast artificial chromosome library construction

Sung Sick Woo; Vipin K. Rastogi; Hong Bin Zhang; Andrew H. Paterson; Keith F. Schertz; Rod A. Wing

doi:10.1007/BF02668396

Isolation of megabase-size DNA from sorghum and applications for physical mapping and bacterial and yeast artificial chromosome library construction

Sung Sick Woo, Vipin K. Rastogi, Hong Bin Zhang, Andrew H. Paterson, Keith F. Schertz, Rod A. Wing

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A method was developed for the isolation of megabase-size DNA from Sorghum bicolor. Sorghum protoplasts were isolated from young leaf tissue, embedded in an agarose matrix as microbeads or plugs, followed by cell lysis and protein degradation. The DNA prepared by this method was larger than 1 Mb in size and readily digestible with restriction enzymes. The DNA was shown to be suitable for physical mapping, and was successfully used for the construction of BAC and YAC libraries.

Original language	English (US)
Pages (from-to)	82-94
Number of pages	13
Journal	Plant Molecular Biology Reporter
Volume	13
Issue number	1
DOIs	https://doi.org/10.1007/BF02668396
State	Published - Mar 1995

Keywords

Megabase-size DNA
Physical mapping
Sorghum bicolor
YAC and BAC cloning

ASJC Scopus subject areas

Molecular Biology
Plant Science

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10.1007/BF02668396

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title = "Isolation of megabase-size DNA from sorghum and applications for physical mapping and bacterial and yeast artificial chromosome library construction",

abstract = "A method was developed for the isolation of megabase-size DNA from Sorghum bicolor. Sorghum protoplasts were isolated from young leaf tissue, embedded in an agarose matrix as microbeads or plugs, followed by cell lysis and protein degradation. The DNA prepared by this method was larger than 1 Mb in size and readily digestible with restriction enzymes. The DNA was shown to be suitable for physical mapping, and was successfully used for the construction of BAC and YAC libraries.",

keywords = "Megabase-size DNA, Physical mapping, Sorghum bicolor, YAC and BAC cloning",

author = "Woo, {Sung Sick} and Rastogi, {Vipin K.} and Zhang, {Hong Bin} and Paterson, {Andrew H.} and Schertz, {Keith F.} and Wing, {Rod A.}",

year = "1995",

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T1 - Isolation of megabase-size DNA from sorghum and applications for physical mapping and bacterial and yeast artificial chromosome library construction

AU - Woo, Sung Sick

AU - Rastogi, Vipin K.

AU - Zhang, Hong Bin

AU - Paterson, Andrew H.

AU - Schertz, Keith F.

AU - Wing, Rod A.

PY - 1995/3

Y1 - 1995/3

N2 - A method was developed for the isolation of megabase-size DNA from Sorghum bicolor. Sorghum protoplasts were isolated from young leaf tissue, embedded in an agarose matrix as microbeads or plugs, followed by cell lysis and protein degradation. The DNA prepared by this method was larger than 1 Mb in size and readily digestible with restriction enzymes. The DNA was shown to be suitable for physical mapping, and was successfully used for the construction of BAC and YAC libraries.

AB - A method was developed for the isolation of megabase-size DNA from Sorghum bicolor. Sorghum protoplasts were isolated from young leaf tissue, embedded in an agarose matrix as microbeads or plugs, followed by cell lysis and protein degradation. The DNA prepared by this method was larger than 1 Mb in size and readily digestible with restriction enzymes. The DNA was shown to be suitable for physical mapping, and was successfully used for the construction of BAC and YAC libraries.

KW - Megabase-size DNA

KW - Physical mapping

KW - Sorghum bicolor

KW - YAC and BAC cloning

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ER -

Isolation of megabase-size DNA from sorghum and applications for physical mapping and bacterial and yeast artificial chromosome library construction

Abstract

Keywords

ASJC Scopus subject areas

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