Label-free detection of NAPPA: Surface plasmon resonance

Manuel Fuentes; Sanjeeva Svrivastava; Nirosahan Ramachandran; Eugenie Hainsworth; Josh LaBaer

doi:10.4032/9789814267779

Label-free detection of NAPPA: Surface plasmon resonance

Manuel Fuentes
, Sanjeeva Svrivastava
, Nirosahan Ramachandran
, Eugenie Hainsworth
, Josh LaBaer

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Our research is focused at coupling to the implementation of Nucleic Acid Programmable Protein Array (NAPPA) technology to real-time label-free SPR-based detection. Here, we show the combination of both technologies that together could lead to the ability to measure binding events in real-time for many protein interactions simultaneously using a label-free technology. This could revolutionize the study of protein interactions networks by enabling quantitative comparisons of binding affinities across many molecular species, as well determining the kinetics rates of binding and release.

Original language	English (US)
Title of host publication	Functional Proteomics and Nanotechnology-Based Microarrays
Publisher	Pan Stanford Publishing Pte. Ltd.
Pages	287-303
Number of pages	17
ISBN (Print)	9789814267762
DOIs	https://doi.org/10.4032/9789814267779
State	Published - Jun 1 2010
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.4032/9789814267779

Cite this

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AU - Fuentes, Manuel

AU - Svrivastava, Sanjeeva

AU - Ramachandran, Nirosahan

AU - Hainsworth, Eugenie

AU - LaBaer, Josh

PY - 2010/6/1

Y1 - 2010/6/1

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AB - Our research is focused at coupling to the implementation of Nucleic Acid Programmable Protein Array (NAPPA) technology to real-time label-free SPR-based detection. Here, we show the combination of both technologies that together could lead to the ability to measure binding events in real-time for many protein interactions simultaneously using a label-free technology. This could revolutionize the study of protein interactions networks by enabling quantitative comparisons of binding affinities across many molecular species, as well determining the kinetics rates of binding and release.

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EP - 303

BT - Functional Proteomics and Nanotechnology-Based Microarrays

PB - Pan Stanford Publishing Pte. Ltd.

ER -

Label-free detection of NAPPA: Surface plasmon resonance

Abstract

ASJC Scopus subject areas

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