Measurement of Cell Death in Mammalian Cells

Brian S. Cummings; Rick G. Schnellmann

doi:10.1002/cpz1.210

Measurement of Cell Death in Mammalian Cells

Brian S. Cummings, Rick G. Schnellmann

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20 Scopus citations

Abstract

Methods for assessing mammalian cell death are presented in this article, which is divided into six sections: (1) a brief overview of cytotoxicity and pathways of cell death; (2) a method to measure cell death using lactate dehydrogenase (LDH) release as a marker of membrane integrity; (3) a flow cytometry method that simultaneously measures two types of cell death, necrosis and apoptosis; (4) use of fluorescence microscopy and nuclear morphology to assess apoptosis and necrosis; (5) the use of multi-well plates and high-content analysis imaging systems to assess nuclear morphology; and (6) a discussion of the use of cytotoxicity assays to determine the mechanisms of cell death.

Original language	English (US)
Article number	e210
Journal	Current Protocols
Volume	1
Issue number	8
DOIs	https://doi.org/10.1002/cpz1.210
State	Published - Aug 2021

Keywords

apoptosis
cytotoxicity
necrosis

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
Pharmacology, Toxicology and Pharmaceutics(all)
General Neuroscience
General Immunology and Microbiology
Health Informatics
Medical Laboratory Technology
General Medicine

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10.1002/cpz1.210

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title = "Measurement of Cell Death in Mammalian Cells",

abstract = "Methods for assessing mammalian cell death are presented in this article, which is divided into six sections: (1) a brief overview of cytotoxicity and pathways of cell death; (2) a method to measure cell death using lactate dehydrogenase (LDH) release as a marker of membrane integrity; (3) a flow cytometry method that simultaneously measures two types of cell death, necrosis and apoptosis; (4) use of fluorescence microscopy and nuclear morphology to assess apoptosis and necrosis; (5) the use of multi-well plates and high-content analysis imaging systems to assess nuclear morphology; and (6) a discussion of the use of cytotoxicity assays to determine the mechanisms of cell death.",

keywords = "apoptosis, cytotoxicity, necrosis",

author = "Cummings, {Brian S.} and Schnellmann, {Rick G.}",

note = "Funding Information: This work was supported by the Department of Defense Spinal Cord Injury Research Program [SC180122] and the Biomedical Laboratory Research and Development Program of the Department of Veterans Affairs BX 005218, BX 004868, RX 003224, BX 000851 to RGS. The authors would like to thank Drs. Laura J.C. Bolchoz, Xiuli Liu, Terry Van Vleet, and David Arrington for their contributions to both the technical aspects and the proofreading of the original manuscript. Publisher Copyright: {\textcopyright} 2021 Wiley Periodicals LLC",

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month = aug,

doi = "10.1002/cpz1.210",

language = "English (US)",

volume = "1",

journal = "Current Protocols",

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AU - Schnellmann, Rick G.

N1 - Funding Information: This work was supported by the Department of Defense Spinal Cord Injury Research Program [SC180122] and the Biomedical Laboratory Research and Development Program of the Department of Veterans Affairs BX 005218, BX 004868, RX 003224, BX 000851 to RGS. The authors would like to thank Drs. Laura J.C. Bolchoz, Xiuli Liu, Terry Van Vleet, and David Arrington for their contributions to both the technical aspects and the proofreading of the original manuscript. Publisher Copyright: © 2021 Wiley Periodicals LLC

PY - 2021/8

Y1 - 2021/8

N2 - Methods for assessing mammalian cell death are presented in this article, which is divided into six sections: (1) a brief overview of cytotoxicity and pathways of cell death; (2) a method to measure cell death using lactate dehydrogenase (LDH) release as a marker of membrane integrity; (3) a flow cytometry method that simultaneously measures two types of cell death, necrosis and apoptosis; (4) use of fluorescence microscopy and nuclear morphology to assess apoptosis and necrosis; (5) the use of multi-well plates and high-content analysis imaging systems to assess nuclear morphology; and (6) a discussion of the use of cytotoxicity assays to determine the mechanisms of cell death.

AB - Methods for assessing mammalian cell death are presented in this article, which is divided into six sections: (1) a brief overview of cytotoxicity and pathways of cell death; (2) a method to measure cell death using lactate dehydrogenase (LDH) release as a marker of membrane integrity; (3) a flow cytometry method that simultaneously measures two types of cell death, necrosis and apoptosis; (4) use of fluorescence microscopy and nuclear morphology to assess apoptosis and necrosis; (5) the use of multi-well plates and high-content analysis imaging systems to assess nuclear morphology; and (6) a discussion of the use of cytotoxicity assays to determine the mechanisms of cell death.

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KW - cytotoxicity

KW - necrosis

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DO - 10.1002/cpz1.210

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JO - Current Protocols

JF - Current Protocols

IS - 8

M1 - e210

ER -

Measurement of Cell Death in Mammalian Cells

Abstract

Keywords

ASJC Scopus subject areas

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