@article{7fb9d44cc99e4384a170f02cb0218f11,
title = "Mimicry of Canonical Translation Elongation Underlies Alanine Tail Synthesis in RQC",
abstract = "Incompletely made nascent chains are tagged for proteolysis by ribosome-associated quality control (RQC). In bacterial RQC, this is mediated by C-terminal alanine tailing. Filbeck et al. elucidate the structural basis for alanine tailing using cryo-EM, discover Hsp15/RqcP as an essential factor, and reveal that C-terminal tailing follows similar principles to canonical translation elongation.",
keywords = "Hsp15, RQC, RqcH, RqcP, SsrA, alanine tailing, cryo-EM, ribosome-associated quality control, ribosomes, translation elongation",
author = "Sebastian Filbeck and Federico Cerullo and Helge Paternoga and George Tsaprailis and Joazeiro, {Claudio A.P.} and Stefan Pfeffer",
note = "Funding Information: The authors thank Jeff Errington (Newcastle University, UK) for FtsZ antibody, Erik Zupa (ZMBH, Heidelberg) for help with atomic modeling, Svenja Beenders for help with experiments, and Sylvia Kreger and Haifei Xu for technical assistance. S.P. acknowledges access to the infrastructure of the Cryo-EM Network at the Heidelberg University (HDcryoNET) and support by G{\"o}tz Hofhaus (Bioquant). S.P. also acknowledges the services SDS@hd and bwHPC supported by the Ministry of Science, Research and the Arts Baden-W{\"u}rttemberg, as well as the German Research Foundation (INST 35/1314-1 FUGG and INST 35/1134-1 FUGG). Work in the Joazeiro laboratory is supported in part by the Deutsche Forschungsgemeinschaft (DFG) (SFB1036) and by the National Institute of Neurological Disorders and Stroke (NINDS) of the NIH (R01 NS102414). S.F. performed all cryo-EM and structure analysis work. F.C. performed all biochemical and molecular genetic work, except purification of samples for cryo-EM, which was performed by H.P. G.T. performed mass spectrometry analyses. S.P. and C.A.P.J. designed studies, conceived and supervised the project, and wrote the manuscript with contributions from S.F. F.C. and H.P. All authors commented on the manuscript. The authors declare no competing interests. Funding Information: The authors thank Jeff Errington (Newcastle University, UK) for FtsZ antibody, Erik Zupa (ZMBH, Heidelberg) for help with atomic modeling, Svenja Beenders for help with experiments, and Sylvia Kreger and Haifei Xu for technical assistance. S.P. acknowledges access to the infrastructure of the Cryo-EM Network at the Heidelberg University (HDcryoNET) and support by G{\"o}tz Hofhaus (Bioquant) . S.P. also acknowledges the services SDS@hd and bwHPC supported by the Ministry of Science, Research and the Arts Baden-W{\"u}rttemberg , as well as the German Research Foundation ( INST 35/1314-1 FUGG and INST 35/1134-1 FUGG ). Work in the Joazeiro laboratory is supported in part by the Deutsche Forschungsgemeinschaft (DFG) ( SFB1036 ) and by the National Institute of Neurological Disorders and Stroke (NINDS) of the NIH ( R01 NS102414 ). Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2021",
month = jan,
day = "7",
doi = "10.1016/j.molcel.2020.11.001",
language = "English (US)",
volume = "81",
pages = "104--114.e6",
journal = "Molecular cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "1",
}