Molecular cloning of murine sodium-phosphate cotransporter type IIb (Na/P(i)-IIb) gene promoter and characterization of gene structure

Kayo Arima; James F. Collins; Eric R. Hines; Liqun Bai; Fayez K. Ghishan

doi:10.1016/S0167-4781(00)00205-0

Molecular cloning of murine sodium-phosphate cotransporter type IIb (Na/P(i)-IIb) gene promoter and characterization of gene structure

Kayo Arima
, James F. Collins
, Eric R. Hines
, Liqun Bai
, Fayez K. Ghishan

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We report the cloning of the murine Na/P(i)-IIb cotransporter gene, which spans more than 18 kilobases and consists of 12 introns and 13 exons. Three promoter/reporter gene constructs, -159/+73, -429/+73 and -954/+73, showed significant luciferase activity (22-82-fold over background) when transfected into in rat intestinal epithelial (RIE-1) cells. Copyright (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	149-154
Number of pages	6
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1494
Issue number	1-2
DOIs	https://doi.org/10.1016/S0167-4781(00)00205-0
State	Published - Nov 15 2000

Keywords

Intestine
Promoter analysis
Sodium-phosphate transporter

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(00)00205-0

Cite this

Molecular cloning of murine sodium-phosphate cotransporter type IIb (Na/P(i)-IIb) gene promoter and characterization of gene structure. / Arima, Kayo; Collins, James F.; Hines, Eric R. et al.
In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1494, No. 1-2, 15.11.2000, p. 149-154.

Research output: Contribution to journal › Article › peer-review

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title = "Molecular cloning of murine sodium-phosphate cotransporter type IIb (Na/P(i)-IIb) gene promoter and characterization of gene structure",

abstract = "We report the cloning of the murine Na/P(i)-IIb cotransporter gene, which spans more than 18 kilobases and consists of 12 introns and 13 exons. Three promoter/reporter gene constructs, -159/+73, -429/+73 and -954/+73, showed significant luciferase activity (22-82-fold over background) when transfected into in rat intestinal epithelial (RIE-1) cells. Copyright (C) 2000 Elsevier Science B.V.",

keywords = "Intestine, Promoter analysis, Sodium-phosphate transporter",

author = "Kayo Arima and Collins, \{James F.\} and Hines, \{Eric R.\} and Liqun Bai and Ghishan, \{Fayez K.\}",

note = "Funding Information: This study was supported by NIH Grant R37DK33209 and the W.M. Keck Foundation.",

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AU - Arima, Kayo

AU - Collins, James F.

AU - Hines, Eric R.

AU - Bai, Liqun

AU - Ghishan, Fayez K.

N1 - Funding Information: This study was supported by NIH Grant R37DK33209 and the W.M. Keck Foundation.

PY - 2000/11/15

Y1 - 2000/11/15

N2 - We report the cloning of the murine Na/P(i)-IIb cotransporter gene, which spans more than 18 kilobases and consists of 12 introns and 13 exons. Three promoter/reporter gene constructs, -159/+73, -429/+73 and -954/+73, showed significant luciferase activity (22-82-fold over background) when transfected into in rat intestinal epithelial (RIE-1) cells. Copyright (C) 2000 Elsevier Science B.V.

AB - We report the cloning of the murine Na/P(i)-IIb cotransporter gene, which spans more than 18 kilobases and consists of 12 introns and 13 exons. Three promoter/reporter gene constructs, -159/+73, -429/+73 and -954/+73, showed significant luciferase activity (22-82-fold over background) when transfected into in rat intestinal epithelial (RIE-1) cells. Copyright (C) 2000 Elsevier Science B.V.

KW - Intestine

KW - Promoter analysis

KW - Sodium-phosphate transporter

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EP - 154

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 1-2

ER -

Molecular cloning of murine sodium-phosphate cotransporter type IIb (Na/P(i)-IIb) gene promoter and characterization of gene structure

Abstract

Keywords

ASJC Scopus subject areas

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