Abstract
In order to understand the distribution of endogenous fluorescence in the ovary, ovarian biopsies were maintained with a viable tissue imaging system and characterized with multiphoton imaging. It was imperative to maintain a stable in vitro environment so that tissue images could provide accurate correlative data for in vivo spectroscopic measurements. Evaluating tissue viability in real time poses a difficult task given that viability assays are tailored for cell culture. The focus of this study was to design a robust in vitro imaging chamber for assessment of ovarian autofluorescence and simple, reliable viability assays for tissue status monitoring.
| Original language | English (US) |
|---|---|
| Article number | 586002 |
| Pages (from-to) | 1-8 |
| Number of pages | 8 |
| Journal | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 5860 |
| State | Published - 2005 |
| Event | Confocal, Multiphoton, and Nonlinear Microscopic Imaging II - Munich, Germany Duration: Jun 12 2005 → Jun 16 2005 |
Keywords
- In vitro imaging
- Multiphoton microscopy
- Tissue diagnostics
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging