Organotypic 3D cell culture models: Using the rotating wall vessel to study hostĝ€"pathogen interactions

Jennifer Barrila; Andrea L. Radtke; Auŕlie Crabbé; Shameema F. Sarker; Melissa M. Herbst-Kralovetz; C. Mark Ott; Cheryl Nickerson

doi:10.1038/nrmicro2423

Organotypic 3D cell culture models: Using the rotating wall vessel to study hostĝ€"pathogen interactions

Jennifer Barrila, Andrea L. Radtke, Auŕlie Crabbé, Shameema F. Sarker, Melissa M. Herbst-Kralovetz, C. Mark Ott, Cheryl Nickerson

Research output: Contribution to journal › Review article › peer-review

241 Scopus citations

Abstract

Appropriately simulating the three-dimensional (3D) environment in which tissues normally develop and function is crucial for engineering in vitro models that can be used for the meaningful dissection of hostĝ€" pathogen interactions. This Review highlights how the rotating wall vessel bioreactor has been used to establish 3D hierarchical models that range in complexity from a single cell type to multicellular co-culture models that recapitulate the 3D architecture of tissues observed in vivo. The application of these models to the study of infectious diseases is discussed.

Original language	English (US)
Pages (from-to)	791-801
Number of pages	11
Journal	Nature Reviews Microbiology
Volume	8
Issue number	11
DOIs	https://doi.org/10.1038/nrmicro2423
State	Published - Nov 2010

ASJC Scopus subject areas

Microbiology
General Immunology and Microbiology
Infectious Diseases

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10.1038/nrmicro2423

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abstract = "Appropriately simulating the three-dimensional (3D) environment in which tissues normally develop and function is crucial for engineering in vitro models that can be used for the meaningful dissection of hostĝ€{"} pathogen interactions. This Review highlights how the rotating wall vessel bioreactor has been used to establish 3D hierarchical models that range in complexity from a single cell type to multicellular co-culture models that recapitulate the 3D architecture of tissues observed in vivo. The application of these models to the study of infectious diseases is discussed.",

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AU - Sarker, Shameema F.

AU - Herbst-Kralovetz, Melissa M.

AU - Ott, C. Mark

AU - Nickerson, Cheryl

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AB - Appropriately simulating the three-dimensional (3D) environment in which tissues normally develop and function is crucial for engineering in vitro models that can be used for the meaningful dissection of hostĝ€" pathogen interactions. This Review highlights how the rotating wall vessel bioreactor has been used to establish 3D hierarchical models that range in complexity from a single cell type to multicellular co-culture models that recapitulate the 3D architecture of tissues observed in vivo. The application of these models to the study of infectious diseases is discussed.

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Organotypic 3D cell culture models: Using the rotating wall vessel to study hostĝ€"pathogen interactions

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ASJC Scopus subject areas

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