TY - JOUR
T1 - Purpose, Partnership, and Possibilities
T2 - The Implementation of the Dog Aging Project Biobank
AU - Mouttham, Lara
AU - Castelhano, Marta G.
AU - Akey, Joshua M.
AU - Benton, Brooke
AU - Borenstein, Elhanan
AU - Castelhano, Marta G.
AU - Coleman, Amanda E.
AU - Creevy, Kate E.
AU - Crowder, Kyle
AU - Dunbar, Matthew D.
AU - Ernst, Holley R.
AU - Fajt, Virginia R.
AU - Fitzpatrick, Annette L.
AU - Garrison, Susan J.
AU - Herndon, Reba S.
AU - Jaramilla, Debra
AU - Jeffery, Unity
AU - Jonlin, Erica C.
AU - Kaeberlein, Matt
AU - Karlsson, Elinor K.
AU - Kerr, Kathleen F.
AU - Levine, Jonathan M.
AU - Ma, Jing
AU - McClelland, Robyn L.
AU - Prescott, Jena O.
AU - Promislow, Daniel E.L.
AU - Ruple, Audrey
AU - Schwartz, Stephen M.
AU - Shrager, Sandi
AU - Snyder-Mackler, Noah
AU - Tinkle, Amanda K.
AU - Tolbert, M. Katherine
AU - Urfer, Silvan R.
AU - Wilfond, Benjamin S.
N1 - Publisher Copyright: © The Author(s) 2022.
PY - 2022/1/1
Y1 - 2022/1/1
N2 - Background: Biobanks have been supporting longitudinal prospective and retrospective studies by providing standardized services for the acquisition, transport, processing, storage, and distribution of high-quality biological material and associated data. Here, we describe how the Dog Aging Project (DAP), a large-scale longitudinal study of the domestic dog (Canis familiaris) with translational applications for humans, developed a biobank of canine biospecimens and associated data. Design and methods: This was accomplished by working with the Cornell Veterinary Biobank, the first biobank in the world to receive accreditation to ISO 20387:2018—General Requirements for Biobanking. The biobank research team was involved in the early collection stages of the DAP, contributing to the development of appropriate workflows and processing fit-for-purpose biospecimens. In support of a dynamic strategy for real-time adjustment of processes, a pilot phase was implemented to develop, test, and optimize the biospecimen workflows, followed by an early phase of collection, processing, and banking of specimens from DAP participants. Results: During the pilot and early phases of collection, the DAP Biobank stored 164 aliquots of whole blood, 273 aliquots of peripheral blood mononuclear cells, 130 aliquots of plasma, and 70 aliquots of serum, and extracted high molecular weight genomic DNA suitable for whole-genome sequencing from 109 whole blood specimens. These specimens, along with their associated preanalytical data, have been made available for distribution to researchers. Conclusion: We discuss the challenges and opportunities encountered during the implementation of the DAP Biobank, along with novel strategies for promoting biobanking sustainability such as partnering with a DAP quality assurance manager and a DAP marketing and communication specialist and developing a pilot grant structure to fund small innovative research projects.
AB - Background: Biobanks have been supporting longitudinal prospective and retrospective studies by providing standardized services for the acquisition, transport, processing, storage, and distribution of high-quality biological material and associated data. Here, we describe how the Dog Aging Project (DAP), a large-scale longitudinal study of the domestic dog (Canis familiaris) with translational applications for humans, developed a biobank of canine biospecimens and associated data. Design and methods: This was accomplished by working with the Cornell Veterinary Biobank, the first biobank in the world to receive accreditation to ISO 20387:2018—General Requirements for Biobanking. The biobank research team was involved in the early collection stages of the DAP, contributing to the development of appropriate workflows and processing fit-for-purpose biospecimens. In support of a dynamic strategy for real-time adjustment of processes, a pilot phase was implemented to develop, test, and optimize the biospecimen workflows, followed by an early phase of collection, processing, and banking of specimens from DAP participants. Results: During the pilot and early phases of collection, the DAP Biobank stored 164 aliquots of whole blood, 273 aliquots of peripheral blood mononuclear cells, 130 aliquots of plasma, and 70 aliquots of serum, and extracted high molecular weight genomic DNA suitable for whole-genome sequencing from 109 whole blood specimens. These specimens, along with their associated preanalytical data, have been made available for distribution to researchers. Conclusion: We discuss the challenges and opportunities encountered during the implementation of the DAP Biobank, along with novel strategies for promoting biobanking sustainability such as partnering with a DAP quality assurance manager and a DAP marketing and communication specialist and developing a pilot grant structure to fund small innovative research projects.
KW - Biobanking
KW - canine
KW - fit-for-purpose
KW - longitudinal study
KW - translational
UR - http://www.scopus.com/inward/record.url?scp=85143284192&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85143284192&partnerID=8YFLogxK
U2 - 10.1177/11772719221137217
DO - 10.1177/11772719221137217
M3 - Article
SN - 1177-2719
VL - 17
JO - Biomarker Insights
JF - Biomarker Insights
ER -