Quantitative analysis of volatile halothane metabolites in biological tissues by gas chromatography

R. M. Maiorino; I. G. Sipes; A. J. Gandolfi; B. R. Brown

doi:10.1016/S0378-4347(00)81572-8

Quantitative analysis of volatile halothane metabolites in biological tissues by gas chromatography

R. M. Maiorino, I. G. Sipes, A. J. Gandolfi, B. R. Brown

Research output: Contribution to journal › Article › peer-review

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Abstract

A simple and sensitive gas chromatographic method for the determination of 2-chloro-1, 1-difluoroethylene (CDE) and 2-chloro-1,1,1-trifluoroethane (CTE), two highly volatile metabolites of halothane, in blood, liver and isolated hepatic microscomes is described. The entire head-space in equilibrium with a known volume or weight of the sample is injected into the gas chromatograph equipped with a flame ionization detector. Quantification is accomplished with standards prepared by fortifying blank samples with known concentrations of CDE and CTE which are treated under the same conditions as the samples. Detection limits for CDE and CTE were 2 pmole/ml in blood and 10 pmole/g in liver and the mean relative standard deviations are no greater than ± 6% except for CTE in hepatic microsomes (± 9%). A preliminary study of blood CDE and CTE levels in humans anesthetized with halothane is reported.

Original language	English (US)
Pages (from-to)	63-72
Number of pages	10
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	164
Issue number	1
DOIs	https://doi.org/10.1016/S0378-4347(00)81572-8
State	Published - Sep 11 1979

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General Chemistry

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title = "Quantitative analysis of volatile halothane metabolites in biological tissues by gas chromatography",

abstract = "A simple and sensitive gas chromatographic method for the determination of 2-chloro-1, 1-difluoroethylene (CDE) and 2-chloro-1,1,1-trifluoroethane (CTE), two highly volatile metabolites of halothane, in blood, liver and isolated hepatic microscomes is described. The entire head-space in equilibrium with a known volume or weight of the sample is injected into the gas chromatograph equipped with a flame ionization detector. Quantification is accomplished with standards prepared by fortifying blank samples with known concentrations of CDE and CTE which are treated under the same conditions as the samples. Detection limits for CDE and CTE were 2 pmole/ml in blood and 10 pmole/g in liver and the mean relative standard deviations are no greater than ± 6% except for CTE in hepatic microsomes (± 9%). A preliminary study of blood CDE and CTE levels in humans anesthetized with halothane is reported.",

author = "Maiorino, {R. M.} and Sipes, {I. G.} and Gandolfi, {A. J.} and Brown, {B. R.}",

note = "Funding Information: This investigation was supported by grant l-ROl-CA21820-01 National Institutes of Health, Bethesda, Md. The mass spectrometic of Sai Chang, Ph.D. and the excellent technical assistance of Dale S. Matthew Miller are gratefully acknowledged.",

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doi = "10.1016/S0378-4347(00)81572-8",

language = "English (US)",

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AU - Maiorino, R. M.

AU - Sipes, I. G.

AU - Gandolfi, A. J.

AU - Brown, B. R.

N1 - Funding Information: This investigation was supported by grant l-ROl-CA21820-01 National Institutes of Health, Bethesda, Md. The mass spectrometic of Sai Chang, Ph.D. and the excellent technical assistance of Dale S. Matthew Miller are gratefully acknowledged.

PY - 1979/9/11

Y1 - 1979/9/11

N2 - A simple and sensitive gas chromatographic method for the determination of 2-chloro-1, 1-difluoroethylene (CDE) and 2-chloro-1,1,1-trifluoroethane (CTE), two highly volatile metabolites of halothane, in blood, liver and isolated hepatic microscomes is described. The entire head-space in equilibrium with a known volume or weight of the sample is injected into the gas chromatograph equipped with a flame ionization detector. Quantification is accomplished with standards prepared by fortifying blank samples with known concentrations of CDE and CTE which are treated under the same conditions as the samples. Detection limits for CDE and CTE were 2 pmole/ml in blood and 10 pmole/g in liver and the mean relative standard deviations are no greater than ± 6% except for CTE in hepatic microsomes (± 9%). A preliminary study of blood CDE and CTE levels in humans anesthetized with halothane is reported.

AB - A simple and sensitive gas chromatographic method for the determination of 2-chloro-1, 1-difluoroethylene (CDE) and 2-chloro-1,1,1-trifluoroethane (CTE), two highly volatile metabolites of halothane, in blood, liver and isolated hepatic microscomes is described. The entire head-space in equilibrium with a known volume or weight of the sample is injected into the gas chromatograph equipped with a flame ionization detector. Quantification is accomplished with standards prepared by fortifying blank samples with known concentrations of CDE and CTE which are treated under the same conditions as the samples. Detection limits for CDE and CTE were 2 pmole/ml in blood and 10 pmole/g in liver and the mean relative standard deviations are no greater than ± 6% except for CTE in hepatic microsomes (± 9%). A preliminary study of blood CDE and CTE levels in humans anesthetized with halothane is reported.

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Quantitative analysis of volatile halothane metabolites in biological tissues by gas chromatography

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