Rotavirus stability and inactivation

The stability of the infectivity of Simian rotavirus, SAII, has been analysed and compared to the stability of reovirus type I. SAII infectivity was stable to freeze-thawing, sonication, incubation at 25°C overnight or at 37°C for 1 h and to treatment with acid, ether, chloroform and Genetron. In contrast to reovirus, the infectivity of SAII was more rapidly inactivated by heating at 50°C. SAII infectivity was inactivated above pH 10.0 and by heating at 50°C in 2 M-MgCl₂, but was stabilized by heating in 2 M-MgSO₄; reovirus I infectivity was enhanced by heating in MgCl₂. Both SAII and reovirus I were inactivated by freezing in MgCl₂. These results show that rotaviruses and reoviruses can be distinguished by their patterns of inactivation by physical and chemical agents.