TY - JOUR
T1 - Secondary metabolites from hypocrealean entomopathogenic fungi
T2 - Genomics as a tool to elucidate the encoded parvome
AU - Zhang, Liwen
AU - Yue, Qun
AU - Wang, Chen
AU - Xu, Yuquan
AU - Molnár, István
N1 - Funding Information: Work in the authors' laboratories are supported by National Transgenic Major Program of China (2019ZX08010-004 to Q. Y.), the National Key Research and Development Program of China (2018YFA0901800 to Y. X.); the National Natural Science Foundation of China (31870076 and 31570093 to Y. X., 31500079 to L. Z.); the USDA National Institute of Food and Agriculture Hatch project (ARZT-1361640-H12-224 to I. M.); the Higher Education Institutional Excellence Program of the Ministry of Human Capacities in Hungary (NKFIH-1150-6/2019 to I. M.); the U.S. National Institutes of Health (NIGMS 5R01GM114418 to I. M.); and the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP to Y. X. and L. Z.). The authors thank Dr Richard A. Humber (USDA ARS, Emeritus) for helpful discussions on the taxonomy of "P. hepiali" FENG. Funding Information: Work in the authors' laboratories are supported by National Transgenic Major Program of China (2019ZX08010-004 to Q. Y.), the National Key Research and Development Program of China (2018YFA0901800 to Y. X.); the National Natural Science Foundation of China (31870076 and 31570093 to Y. X., 31500079 to L. Z.); the USDA National Institute of Food and Agriculture Hatch project (ARZT-1361640-H12-224 to I. M.); the Higher Education Institutional Excellence Program of the Ministry of Human Capacities in Hungary (NKFIH-1150-6/2019 to I. M.); the U.S. National Institutes of Health (NIGMS 5R01GM114418 to I. M.); and the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP to Y. X. and L. Z.). The authors thank Dr Richard A. Humber (USDA ARS, Emeritus) for helpful discussions on the taxonomy of “P. hepiali” FENG. Publisher Copyright: © 2020 The Royal Society of Chemistry.
PY - 2020/9
Y1 - 2020/9
N2 - Hypocrealean entomopathogenic fungi (HEF) produce a large variety of secondary metabolites (SMs) that are prominent virulence factors or mediate various interactions in the native niches of these organisms. Many of these SMs show insecticidal, immune system modulatory, antimicrobial, cytotoxic and other bioactivities of clinical or agricultural significance. Recent advances in whole genome sequencing technologies and bioinformatics have revealed many biosynthetic gene clusters (BGCs) potentially involved in SM production in HEF. Some of these BGCs are now well characterized, with the structures of the cognate product congeners elucidated, and the proposed biosynthetic functions of key enzymes validated. However, the vast majority of HEF BGCs are still not linked to SM products ("orphan"BGCs), including many clusters that are not expressed (silent) under routine laboratory conditions. Thus, investigations into the encoded parvome (the secondary metabolome predicted from the genome) of HEF allows the discovery of BGCs for known SMs; uncovers novel metabolites based on the BGCs; and catalogues the predicted SM biosynthetic potential of these fungi. Herein, we summarize new developments of the field, and survey the polyketide, nonribosomal peptide, terpenoid and hybrid SM BGCs encoded in the currently available 40 HEF genome sequences. Studying the encoded parvome of HEF will increase our understanding of the multifaceted roles that SMs play in biotic and abiotic interactions and will also reveal biologically active SMs that can be exploited for the discovery of human and veterinary drugs or crop protection agents.
AB - Hypocrealean entomopathogenic fungi (HEF) produce a large variety of secondary metabolites (SMs) that are prominent virulence factors or mediate various interactions in the native niches of these organisms. Many of these SMs show insecticidal, immune system modulatory, antimicrobial, cytotoxic and other bioactivities of clinical or agricultural significance. Recent advances in whole genome sequencing technologies and bioinformatics have revealed many biosynthetic gene clusters (BGCs) potentially involved in SM production in HEF. Some of these BGCs are now well characterized, with the structures of the cognate product congeners elucidated, and the proposed biosynthetic functions of key enzymes validated. However, the vast majority of HEF BGCs are still not linked to SM products ("orphan"BGCs), including many clusters that are not expressed (silent) under routine laboratory conditions. Thus, investigations into the encoded parvome (the secondary metabolome predicted from the genome) of HEF allows the discovery of BGCs for known SMs; uncovers novel metabolites based on the BGCs; and catalogues the predicted SM biosynthetic potential of these fungi. Herein, we summarize new developments of the field, and survey the polyketide, nonribosomal peptide, terpenoid and hybrid SM BGCs encoded in the currently available 40 HEF genome sequences. Studying the encoded parvome of HEF will increase our understanding of the multifaceted roles that SMs play in biotic and abiotic interactions and will also reveal biologically active SMs that can be exploited for the discovery of human and veterinary drugs or crop protection agents.
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U2 - 10.1039/d0np00007h
DO - 10.1039/d0np00007h
M3 - Article
C2 - 32211677
SN - 0265-0568
VL - 37
SP - 1164
EP - 1180
JO - Natural Product Reports
JF - Natural Product Reports
IS - 9
ER -